Yue Yingying, Jiang Haitang, Yin Yingying, Zhang Yuqun, Liang Jinfeng, Li Shenghua, Wang Jun, Lu Jianxin, Geng Deqin, Wu Aiqin, Yuan Yonggui
Department of Psychosomatics and Psychiatry, Institute of Psychosomatic Medicine, Zhongda Hospital, Medical School of Southeast University Nanjing, China.
Department of Neurology, Jiangning Nanjing Hospital Nanjing, China.
Front Aging Neurosci. 2016 Dec 27;8:323. doi: 10.3389/fnagi.2016.00323. eCollection 2016.
Previous studies demonstrate that the protein of neuropeptide Y (NPY) is abnormal in depression patients, but the changes of NPY in different types of depression are unclear. This study was aimed to examine protein and mRNA expression levels of NPY in 159 cases with four groups including post-stroke depression (PSD) group, stroke without depression (Non-PSD) group, major depressive disorder (MDD) group and normal control (NC) group. The protein and gene expression analysis were performed by enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction-based methods. One way analysis of variance (ANOVA), chi-square tests and nonparametric test were used to evaluate general characteristics, clinical and biological materials. In order to explore the role of NPY in different types of depression, the partial correlations, binary logistic regression analysis and receiver operating characteristic (ROC) curve were calculated for PSD and MDD groups. There are significant differences of NPY protein ( = 5.167, = 0.002) and mRNA expression levels ([Formula: see text] = 20.541, < 0.001) among four groups. Bonferroni multiple comparisons found that the NPY protein was significantly decreased in PSD ( = -7.133, = 0.002) and Non-PSD group ( = -5.612, = 0.018) compared with NC group. However, contrasted with MDD group, the mRNA expression was increased in PSD and Non-PSD group by nonparametric test (all < 0.05). In binary logistic analyses, NPY mRNA expression was independent predictors of PSD (odds ratio: 1.452, 95% CI, 1.081-1.951, = 0.013). The ROC curve showed NPY mRNA had a general prognostic accuracy (area under the curve: 0.766, 95% CI, 0.656-0.876, < 0.001). This is the first study to explore the distinguishing function of NPY in different types of depression. It will provide help in the identification of different subtypes of depression.
先前的研究表明，抑郁症患者中神经肽Y（NPY）蛋白异常，但不同类型抑郁症中NPY的变化尚不清楚。本研究旨在检测159例患者中NPY的蛋白和mRNA表达水平，这些患者分为四组，包括中风后抑郁症（PSD）组、无抑郁症的中风患者（非PSD）组、重度抑郁症（MDD）组和正常对照组（NC）。采用酶联免疫吸附测定（ELISA）和基于定量聚合酶链反应的方法进行蛋白和基因表达分析。采用单因素方差分析（ANOVA）、卡方检验和非参数检验来评估一般特征、临床和生物学资料。为了探究NPY在不同类型抑郁症中的作用，对PSD组和MDD组进行了偏相关分析、二元逻辑回归分析和受试者工作特征（ROC）曲线分析。四组之间NPY蛋白（F = 5.167，P = 0.002）和mRNA表达水平（[公式：见正文]F = 20.541，P < 0.001）存在显著差异。Bonferroni多重比较发现，与NC组相比，PSD组（t = -7.133，P = 0.002）和非PSD组（t = -5.612，P = 0.018）的NPY蛋白显著降低。然而，与MDD组相比，通过非参数检验发现PSD组和非PSD组的mRNA表达增加（均P < 0.05）。在二元逻辑分析中，NPY mRNA表达是PSD的独立预测因子（比值比：1.452，95%可信区间，1.081 - 1.951，P = 0.013）。ROC曲线显示NPY mRNA具有一般的预后准确性（曲线下面积：0.766，95%可信区间，0.656 - 0.876，P < 0.001）。这是第一项探究NPY在不同类型抑郁症中鉴别功能的研究。它将有助于鉴别不同亚型的抑郁症。
