Suematsu M, Kurose I, Asako H, Miura S, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo.
J Biochem. 1989 Aug;106(2):355-60. doi: 10.1093/oxfordjournals.jbchem.a122857.
Oxyradical-dependent chemiluminescence from granulocytes sticking to venular endothelium was successfully visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF). Intravital photonic image intensifier microscopy revealed that topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on endothelial walls and the subsequent activation of a luminol-dependent photonic burst. Chemilumigenic sites clearly corresponded to the spatial distribution of sticking cells in post-capillary venules. The present findings thus serve as the first demonstration of an intravital oxidative burst of granulocytes on venular endothelium in PAF-induced microcirculatory disturbances.
在用血小板活化因子（PAF）处理的大鼠肠系膜微血管床中，成功观察到了黏附于小静脉内皮细胞的粒细胞产生的氧自由基依赖性化学发光现象。活体光子图像增强显微镜显示，局部应用PAF-乙醚（100 nM）可导致粒细胞显著黏附于内皮细胞壁，并随后激活依赖鲁米诺的光子爆发。化学发光位点与毛细血管后微静脉中黏附细胞的空间分布明显对应。因此，本研究结果首次证明了在PAF诱导的微循环紊乱中，小静脉内皮细胞上粒细胞的活体氧化爆发。
