接触纸试验法检测黄曲霉毒素 B 对赤子爱胜蚓的毒性作用。
Toxicological effects of aflatoxin B on the earthworm Eisenia fetida as determined in a contact paper test.
机构信息
MTA-KE Mycotoxins in the Food Chain Research Group, Kaposvár University, Guba S. 40, Kaposvár, H-7400, Hungary.
Faculty of Agricultural and Environmental Sciences, Kaposvár University, Guba S. 40, Kaposvár, H-7400, Hungary.
出版信息
Mycotoxin Res. 2017 May;33(2):109-112. doi: 10.1007/s12550-017-0269-y. Epub 2017 Jan 12.
In this study, aflatoxin B (AFB) toxicity toward the earthworm Eisenia fetida (Savigny 1826) was evaluated in contact paper test systems containing distilled water and ethanol or 20 to 400 μg/ml of AFB over 72 h of exposure. The results indicated that AFB could induce significant damage to earthworms (coiling, curling, excessive mucus secretion, clitellum swelling) at greater than 75 μg/ml. Moreover, AFB had harmful effects on E. fetida (degenerative changes such as bulging of the clitella regions) at levels higher than 150 μg/ml. The calculated LD was 168.5 μg/ml. These findings confirm that E. fetida and standardized methods based on this organism (OECD 207 1984) are applicable and useful in mycotoxin related toxicity studies.
本研究采用接触纸测试系统，评估黄曲霉毒素 B（AFB）对赤子爱胜蚓（Savigny 1826）的毒性，系统中含有蒸馏水、乙醇以及浓度为 20 至 400μg/ml 的 AFB，暴露时间为 72 小时。结果表明，大于 75μg/ml 的 AFB 可引起蚯蚓明显损伤（卷曲、蜷缩、过度分泌黏液、生殖带肿胀）。此外，高于 150μg/ml 的 AFB 对赤子爱胜蚓有有害影响（生殖带区域隆起等退行性变化）。计算得出的 LD 为 168.5μg/ml。这些发现证实赤子爱胜蚓和基于该生物的标准化方法（OECD 207 1984）适用于与真菌毒素相关的毒性研究，并具有实际应用价值。
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