Williams S C, Grant S G, Reue K, Carrasquillo B, Lusis A J, Kinniburgh A J
Department of Human Genetics, Roswell Park Memorial Institute, Buffalo, New York 14263.
J Biol Chem. 1989 Nov 15;264(32):19009-16.
The levels of apolipoprotein A-IV (apoA-IV) mRNA are regulated by dietary lipid in the liver of both the mouse and rat. Thirteen different inbred mouse strains were fed a high lipid diet, and the effect on apoA-IV liver mRNA levels was examined. It was found that each strain responded in one of two ways. Mice of four strains had higher liver apoA-IV mRNA levels as compared with syngeneic mice fed a normal chow diet. Mice of the other nine strains had decreased liver apoA-IV mRNA levels as compared with syngeneic mice fed a normal chow diet. Using F1 hybrids between mice from BALB/c, C3H, and C57BL/6 and between 129 and C57BL/6, as well as recombinant inbred strains derived from a cross between BALB/c and C57BL/6, we have shown that both the normal level of liver apoA-IV mRNA in the chow-fed mice and the lipid-dependent regulation of apoA-IV mRNA levels are controlled by cis-acting genetic elements. The apoA-IV mRNA levels in mice fed a normal diet varied dramatically among strains, with the largest difference (90-fold) being between the 129/J inbred strain and the C57BL/6J strain. In addition, we have examined the expression of apoA-IV during mouse development. ApoA-IV mRNA is expressed early in mouse liver (16 days postcoitum), whereas others have shown previously that rat liver apoA-IV mRNA is undetectable until 14 days after birth. ApoA-IV mRNA levels in the intestine and apoA-I mRNA levels in the liver and intestine, by contrast, mirror the pattern seen in the rat.
载脂蛋白A-IV（apoA-IV）mRNA的水平在小鼠和大鼠肝脏中受饮食脂质的调节。给13种不同的近交系小鼠喂食高脂饮食，并检测其对apoA-IV肝脏mRNA水平的影响。结果发现，每个品系有两种反应方式之一。与喂食正常食物的同基因小鼠相比，四个品系的小鼠肝脏apoA-IV mRNA水平较高。与喂食正常食物的同基因小鼠相比，其他九个品系的小鼠肝脏apoA-IV mRNA水平降低。利用BALB/c、C3H和C57BL/6品系小鼠之间以及129和C57BL/6品系小鼠之间的F1杂种，以及源自BALB/c和C57BL/6杂交的重组近交系，我们已经表明，喂食普通食物小鼠肝脏中apoA-IV mRNA的正常水平以及apoA-IV mRNA水平的脂质依赖性调节均由顺式作用遗传元件控制。喂食正常饮食的小鼠中，apoA-IV mRNA水平在不同品系间差异极大，最大差异（90倍）存在于129/J近交系和C57BL/6J品系之间。此外，我们检测了apoA-IV在小鼠发育过程中的表达。apoA-IV mRNA在小鼠肝脏发育早期（胚胎期16天）就有表达，而此前其他人已经表明，大鼠肝脏apoA-IV mRNA在出生后14天之前检测不到。相比之下，肠道中apoA-IV mRNA水平以及肝脏和肠道中apoA-I mRNA水平与大鼠中的模式相似。
