Ippolito Joseph A, Krell Ethan S, Cottrell Jessica, Meyer Ryan, Clark Devin, Nguyen Daniel, Sudah Suleiman, Muñoz Maximillian, Lim Elisha, Lin Anthony, Lee Thomas Jae Hoon, O'Connor James Patrick, Benevenia Joseph, Lin Sheldon S
Department of Orthopaedics, Rutgers New Jersey Medical School, 90 Bergen Street, Suite 7300, Newark, New Jersey 07101.
Department of Biological Sciences, Seton Hall University, South Orange, New Jersey.
J Orthop Res. 2017 Oct;35(10):2174-2180. doi: 10.1002/jor.23521. Epub 2017 Mar 8.
This study evaluated the effect of local vanadyl acetylacetonate (VAC), an insulin mimetic agent, upon the early and late parameters of fracture healing in rats using a standard femur fracture model. Mechanical testing, and radiographic scoring were performed, as well as histomorphometry, including percent bone, percent cartilage, and osteoclast numbers. Fractures treated with local 1.5 mg/kg VAC possessed significantly increased mechanical properties compared to controls at 6 weeks post-fracture, including increased torque to failure (15%; p = 0.046), shear modulus (89%; p = 0.043), and shear stress (81%; p = 0.009). The radiographic scoring analysis showed increased cortical bridging at 4 weeks and 6 weeks (119%; p = 0.036 and 209%; p = 0.002) in 1.5 mg/kg VAC treated groups. Histomorphometry of the fracture callus at days 10 and 14 showed increased percent cartilage (121%; p = 0.009 and 45%; p = 0.035) and percent mineralized tissue (66%; p = 0.035 and 58%; p = 0.006) with local VAC treated groups compared to control. Additionally, fewer osteoclasts were observed in the local VAC treated animals as compared to controls at day 14 (0.45% ± 0.29% vs. 0.83% ± 0.36% of callus area; p = 0.032). The results suggest local administration of VAC acts to modulate osteoclast activity and increase percentage of early callus cartilage, ultimately enhancing mechanical properties comparably to non-diabetic animals treated with local VAC. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2174-2180, 2017.
本研究使用标准股骨骨折模型，评估了胰岛素模拟剂乙酰丙酮氧钒（VAC）局部给药对大鼠骨折愈合早期和晚期参数的影响。进行了力学测试、影像学评分以及组织形态计量学分析，包括骨百分比、软骨百分比和破骨细胞数量。与对照组相比，骨折后6周局部给予1.5mg/kg VAC治疗的骨折部位力学性能显著提高，包括破坏扭矩增加（15%；p = 0.046）、剪切模量增加（89%；p = 0.043）和剪应力增加（81%；p = 0.009）。影像学评分分析显示，1.5mg/kg VAC治疗组在4周和6周时皮质桥接增加（分别为119%；p = 0.036和209%；p = 0.002）。与对照组相比，局部VAC治疗组在第10天和第14天骨折痂的组织形态计量学分析显示软骨百分比增加（分别为121%；p = 0.009和45%；p = 0.035）以及矿化组织百分比增加（分别为66%；p = 0.035和58%；p = 0.006）。此外，与对照组相比，在第14天局部VAC治疗的动物中观察到的破骨细胞较少（分别为骨痂面积的0.45%±0.29%和0.83%±0.36%；p = 0.032）。结果表明，局部给予VAC可调节破骨细胞活性并增加早期骨痂软骨百分比，最终与局部给予VAC治疗的非糖尿病动物相比，同等程度地增强力学性能。©2017骨科研究协会。由Wiley Periodicals, Inc.出版。《矫形外科学研究》35:2174 - 2180, 2017。
