房水抗菌活性：局部应用0.5%氯霉素后的体外分析
Aqueous Humor Antimicrobial Activity: In Vitro Analysis after Topical 0.5% Chloramphenicol Application.
作者信息
Cagini Carlo, Dragoni Annalisa, Orsolini Giampaolo, Fiore Tito, Beccasio Alfredo, Spadea Leopoldo, Moretti Amedeo, Mencacci Antonella
机构信息
a Department of Surgery and Biomedical Science , University of Perugia, Ospedale S. Maria della Misericordia , Perugia , Italy.
b Department of Biotechnology and Medical-Surgical Science , "Sapienza" University of Rome , Latina , Italy.
出版信息
Curr Eye Res. 2017 Jun;42(6):847-851. doi: 10.1080/02713683.2016.1256414. Epub 2017 Jan 13.
PURPOSE
To assess aqueous humor antimicrobial activity in vitro after topical 0.5% chloramphenicol application.
MATERIALS AND METHODS
This investigation included 63 eyes from 65 cataract surgery patients. The study group of 48 eyes received preoperatively four topical applications of 0.5% chloramphenicol. The control group of 15 eyes was given no topical applications. Aqueous humor samples were collected for in vitro antimicrobial analysis using Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Pasteurella multocida organisms by means of disk diffusion test.
RESULTS
No inhibition halo was observed around all aqueous humor samples from all chloramphenicol-treated patients, irrespective of the sample quantity added to the paper disks, with no significant difference from aqueous humor from untreated control patients.
CONCLUSIONS
Aqueous humor displayed no bactericidal effect against any of the microorganisms evaluated after topical 0.5% chloramphenicol application.
目的
评估局部应用0.5%氯霉素后房水的体外抗菌活性。
材料与方法
本研究纳入了65例白内障手术患者的63只眼。48只眼的研究组术前接受了四次0.5%氯霉素的局部应用。15只眼的对照组未进行局部应用。通过纸片扩散试验，收集房水样本，使用肺炎链球菌、流感嗜血杆菌、卡他莫拉菌和多杀巴斯德菌进行体外抗菌分析。
结果
在所有接受氯霉素治疗患者的房水样本中，无论添加到纸片上的样本量如何，均未观察到抑菌圈，与未治疗的对照患者的房水无显著差异。
结论
局部应用0.5%氯霉素后，房水对所评估的任何微生物均无杀菌作用。
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