Srivastava Amrita, Singh Anumeha, Singh Satya S, Mishra Arun K
a Laboratory of Microbial Genetics, Department of Botany , Banaras Hindu University , Varanasi , India.
b Life Science Programme , Central University of South Bihar , Patna , India.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2017 Apr 16;52(5):420-428. doi: 10.1080/10934529.2016.1270672. Epub 2017 Jan 13.
An appreciation of comparative microbial survival is most easily done while evaluating their adaptive strategies during stress. In the present experiment, antioxidative and whole cell proteome variations based on spectrophotometric analysis and SDS-PAGE and 2-dimensional gel electrophoresis have been analysed among salt-tolerant and salt-sensitive Frankia strains. This is the first report of proteomic basis underlying salt tolerance in these newly isolated Frankia strains from Hippophae salicifolia D. Don. Salt-tolerant strain HsIi10 shows higher increment in the contents of superoxide dismutase, catalase and ascorbate peroxidase as compared to salt-sensitive strain HsIi8. Differential 2-DGE profile has revealed differential profiles for salt-tolerant and salt-sensitive strains. Proteomic confirmation of salt tolerance in the strains with inbuilt efficiency of thriving in nitrogen-deficient locales is a definite advantage for these microbes. This would be equally beneficial for improvement of soil nitrogen status. Efficient protein regulation in HsIi10 suggests further exploration for its potential use as biofertilizer in saline soils.
在评估微生物在压力下的适应策略时，最容易了解它们的相对生存情况。在本实验中，基于分光光度分析、SDS-PAGE和二维凝胶电泳，分析了耐盐和盐敏感的弗兰克氏菌菌株之间的抗氧化和全细胞蛋白质组变化。这是关于从沙棘中分离出的这些新的弗兰克氏菌菌株耐盐性的蛋白质组学基础的首次报道。与盐敏感菌株HsIi8相比，耐盐菌株HsIi10的超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶含量增加更高。差异二维凝胶电泳图谱揭示了耐盐和盐敏感菌株的差异图谱。对这些在缺氮环境中具有内在生长效率的菌株的耐盐性进行蛋白质组学确认，对这些微生物来说是一个明确的优势。这对改善土壤氮素状况同样有益。HsIi10中有效的蛋白质调节表明，进一步探索其在盐渍土壤中作为生物肥料的潜在用途是有意义的。
