Itagaki S, McGeer P L, Akiyama H, Zhu S, Selkoe D
University of British Columbia, Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada.
J Neuroimmunol. 1989 Oct;24(3):173-82. doi: 10.1016/0165-5728(89)90115-x.
The relationship of microglia and astrocytes to deposits of beta-amyloid protein (BAP) was studied in Alzheimer's disease by immunohistochemistry. BAP was detected in forms varying from diffuse amorphous deposits to compact spherical masses. These latter corresponded in frequency and distribution to senile plaques revealed by Bielschowsky and thioflavine S staining. Approximately half the diffuse deposits had no human leukocyte antigen (HLA)-DR-positive reactive microglia associated with them while nearly all compact deposits had single or multiple HLA-DR-positive reactive microglia embedded in their core. Electron microscopy showed these reactive microglia to be in intimate contact with amyloid fibrils. These data suggest that amyloid deposition may precede the activation of microglia.
通过免疫组织化学方法研究了阿尔茨海默病中微胶质细胞和星形胶质细胞与β-淀粉样蛋白（BAP）沉积的关系。检测到的BAP形式多样，从弥漫性无定形沉积物到致密球形团块。后者在频率和分布上与 Bielschowsky 染色和硫黄素 S 染色显示的老年斑相对应。大约一半的弥漫性沉积物没有与之相关的人类白细胞抗原（HLA）-DR阳性反应性微胶质细胞，而几乎所有致密沉积物的核心都嵌入了单个或多个HLA-DR阳性反应性微胶质细胞。电子显微镜显示这些反应性微胶质细胞与淀粉样纤维紧密接触。这些数据表明淀粉样蛋白沉积可能先于微胶质细胞的激活。
