Cannella B, Raine C S
Department of Pathology (Neuropathology), Albert Einstein College of Medicine, Bronx, NY 10461.
J Neuroimmunol. 1989 Oct;24(3):239-48. doi: 10.1016/0165-5728(89)90122-7.
Myelinated cultures of embryonic SJL/J mouse spinal cord tissue have been tested for their ability to express class II major histocompatibility complex (MHC) (Ia) molecules in the presence of two recombinant cytokines, mouse interferon-gamma (IFN-gamma) and human tumor necrosis factor-alpha (TNF-alpha). Both cytokines up-regulated class II MHC in a dose-dependent manner and interacted synergistically to increase Ia when applied together. The principal cell type involved were glial fibrillary acidic protein-positive astrocytes along the surface of the cultures. Immunocytochemistry in combination with light microscope examination of whole mounts and epoxy sections as well as electron microscopy, were used to assess the phenomena. These findings add further weight to previous observations on astrocytes being capable of serving as antigen-presenting cells within the central nervous system.
对胚胎SJL/J小鼠脊髓组织的有髓培养物进行了测试，以检测其在两种重组细胞因子（小鼠干扰素-γ（IFN-γ）和人肿瘤坏死因子-α（TNF-α））存在的情况下表达II类主要组织相容性复合体（MHC）（Ia）分子的能力。两种细胞因子均以剂量依赖性方式上调II类MHC，并且一起应用时相互协同作用以增加Ia。主要涉及的细胞类型是培养物表面的胶质纤维酸性蛋白阳性星形胶质细胞。免疫细胞化学结合对整装标本和环氧树脂切片的光学显微镜检查以及电子显微镜检查，用于评估这些现象。这些发现进一步支持了先前关于星形胶质细胞能够作为中枢神经系统内抗原呈递细胞的观察结果。
