• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

平菇转化中选择标记的发展与改进

A development and an improvement of selectable markers in Pleurotus ostreatus transformation.

作者信息

Matsunaga Yukari, Ando Megumi, Izumitsu Kosuke, Suzuki Kazumi, Honda Yoichi, Irie Toshikazu

机构信息

School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.

Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto Prefecture 606-8502, Japan.

出版信息

J Microbiol Methods. 2017 Mar;134:27-29. doi: 10.1016/j.mimet.2017.01.007. Epub 2017 Jan 10.

DOI:10.1016/j.mimet.2017.01.007
PMID:28087470
Abstract

Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA.

摘要

糙皮侧耳首次使用诺尔丝菌素抗性基因进行转化。转化效率为1.3±0.6个转化子/μg质粒DNA。此外，双丙氨膦抗性基因的转化效率提高到了26.7±11.5个转化子/μg质粒DNA。

相似文献

1
A development and an improvement of selectable markers in Pleurotus ostreatus transformation.平菇转化中选择标记的发展与改进
J Microbiol Methods. 2017 Mar;134:27-29. doi: 10.1016/j.mimet.2017.01.007. Epub 2017 Jan 10.
2
Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus.平菇同担子菌的羧菌灵抗性转化
Curr Genet. 2000 Mar;37(3):209-12. doi: 10.1007/s002940050521.
3
The integrative transformation of Pleurotus ostreatus using bialaphos resistance as a dominant selectable marker.以双丙氨膦抗性作为显性选择标记对平菇进行的整合转化。
Biosci Biotechnol Biochem. 1996 Mar;60(3):472-5. doi: 10.1271/bbb.60.472.
4
Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA.利用单链载体DNA高效转化丝状真菌糙皮侧耳
Appl Microbiol Biotechnol. 2001 May;55(5):563-5. doi: 10.1007/s002530000535.
5
Isolation and transformation of uracil auxotrophs of the edible basidiomycete Pleurotus ostreatus.食用担子菌平菇尿嘧啶营养缺陷型菌株的分离与转化
FEMS Microbiol Lett. 1999 Dec 15;181(2):225-8. doi: 10.1111/j.1574-6968.1999.tb08848.x.
6
Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals.利用香菇甘油醛-3-磷酸脱氢酶（GPD）表达信号将糙皮侧耳稳定转化为潮霉素B抗性。
Appl Microbiol Biotechnol. 2001 Sep;56(5-6):707-9. doi: 10.1007/s002530100651.
7
Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker.利用双丙氨膦抗性作为显性选择标记的黄孢原毛平革菌锰过氧化物酶的同源表达。
Curr Genet. 2003 Sep;43(6):407-14. doi: 10.1007/s00294-003-0418-z. Epub 2003 Jul 3.
8
A highly efficient polyethylene glycol-mediated transformation method for mushrooms.一种用于蘑菇的高效聚乙二醇介导的转化方法。
FEMS Microbiol Lett. 2006 Mar;256(2):203-8. doi: 10.1111/j.1574-6968.2006.00110.x.
9
Marker recycling via 5-fluoroorotic acid and 5-fluorocytosine counter-selection in the white-rot agaricomycete Pleurotus ostreatus.通过5-氟乳清酸和5-氟胞嘧啶反选在白腐木耳平菇中进行标记物回收
Fungal Biol. 2016 Sep;120(9):1146-55. doi: 10.1016/j.funbio.2016.06.011. Epub 2016 Jul 2.
10
Transformation of the edible mushroom Pleurotus ostreatus by particle bombardment.
FEMS Microbiol Lett. 2002 Jun 4;211(2):143-6. doi: 10.1111/j.1574-6968.2002.tb11216.x.

引用本文的文献

1
Review on mushroom mycelium-based products and their production process: from upstream to downstream.基于蘑菇菌丝体的产品及其生产过程综述：从上游到下游
Bioresour Bioprocess. 2025 Jan 10;12(1):3. doi: 10.1186/s40643-024-00836-7.
2
Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences.糙皮侧耳（平菇）作为遗传学、细胞生物学和材料科学的模式蘑菇。
Appl Microbiol Biotechnol. 2024 Feb 19;108(1):217. doi: 10.1007/s00253-024-13034-4.
3
Establishment of an Efficient Polyethylene Glycol (PEG)-Mediated Transformation System in var. Using Comprehensive Optimization and Multiple Endogenous Promoters.
利用综合优化和多个内源启动子在[品种名称]中建立高效的聚乙二醇（PEG）介导的转化系统。
J Fungi (Basel). 2022 Feb 14;8(2):186. doi: 10.3390/jof8020186.
4
A 14-bp stretch plays a critical role in regulating gene expression from β-tubulin promoters of basidiomycetes.一段 14 个碱基的序列在调控担子菌β-微管蛋白启动子的基因表达中起着关键作用。
Curr Genet. 2020 Feb;66(1):217-228. doi: 10.1007/s00294-019-01014-5. Epub 2019 Jul 6.