将Argonaute靶向至染色质。
Targeting Argonaute to chromatin.
作者信息
Wendte Jered M, Pikaard Craig S
机构信息
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, Indiana 47405, USA.
出版信息
Genes Dev. 2016 Dec 15;30(24):2649-2650. doi: 10.1101/gad.294900.116.
Abstract
In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to complementary loci, resulting in transcriptional gene silencing. Multiple lines of evidence indicate that siRNAs base-pair with longer RNAs produced at target loci, but the possibility that siRNAs base-pair directly with DNA remains an attractive hypothesis. In a recent study, Shimada et al. (pp. 2571-2580) conducted experiments that address these alternative hypotheses, yielding additional evidence that fission yeast siRNA-Argonaute silencing complexes are recruited to target loci exclusively via interactions with nascent transcripts.
摘要
在许多真核生物中，与AGO蛋白结合的小干扰RNA（siRNA）引导染色质修饰酶作用于互补位点，导致转录基因沉默。多条证据表明，siRNA与靶位点产生的较长RNA进行碱基配对，但siRNA直接与DNA进行碱基配对的可能性仍然是一个引人关注的假说。在最近的一项研究中，岛田等人（第2571 - 2580页）进行了实验来验证这些替代假说，得出了更多证据，即裂殖酵母siRNA-AGO沉默复合体仅通过与新生转录本的相互作用被招募到靶位点。
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本文引用的文献
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2
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Biochim Biophys Acta Gene Regul Mech. 2017 Jan;1860(1):140-148. doi: 10.1016/j.bbagrm.2016.08.004. Epub 2016 Aug 10.
3
RNA-mediated epigenetic regulation of gene expression.RNA 介导的基因表达表观遗传调控。
Nat Rev Genet. 2015 Feb;16(2):71-84. doi: 10.1038/nrg3863. Epub 2015 Jan 2.
4
SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation.SRA- 和 SET 结构域蛋白将 RNA 聚合酶 V 的占据与 DNA 甲基化联系起来。
Nature. 2014 Mar 6;507(7490):124-128. doi: 10.1038/nature12931. Epub 2014 Jan 22.
5
A transcription fork model for Pol IV and Pol V-dependent RNA-directed DNA methylation.一种依赖于RNA聚合酶IV和RNA聚合酶V的RNA指导的DNA甲基化的转录叉模型。
Cold Spring Harb Symp Quant Biol. 2012;77:205-12. doi: 10.1101/sqb.2013.77.014803. Epub 2013 Apr 8.
6
Interplay among RNA polymerases II, IV and V in RNA-directed DNA methylation at a low copy transgene locus in Arabidopsis thaliana.RNA 聚合酶 II、IV 和 V 在拟南芥低拷贝转基因座的 RNA 指导的 DNA 甲基化中的相互作用。
Plant Mol Biol. 2013 May;82(1-2):85-96. doi: 10.1007/s11103-013-0041-4. Epub 2013 Mar 20.
7
DNA-RNA hybrid formation mediates RNAi-directed heterochromatin formation.DNA-RNA 杂交体的形成介导了 RNAi 指导的异染色质形成。
Genes Cells. 2012 Mar;17(3):218-33. doi: 10.1111/j.1365-2443.2012.01583.x. Epub 2012 Jan 27.
8
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9
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Science. 2006 Aug 25;313(5790):1134-7. doi: 10.1126/science.1128813.
10
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Cell. 2006 Jun 2;125(5):873-86. doi: 10.1016/j.cell.2006.04.025.
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