Rowe Walter F, Marginean Ioan, Carnes Stephanie, Lurie Ira S
Department of Forensic Sciences, The George Washington University, 2100 Foxhall Road, NW, Somers Hall, Washington, DC, 20007, USA.
Drug Test Anal. 2017 Oct;9(10):1512-1521. doi: 10.1002/dta.2163. Epub 2017 Mar 15.
The utility of diode array ultraviolet (UV) detection for aiding in the identification of synthetic cathinones, including different sub-classes and positional isomers is presented. For 35 synthetic cathinones, unique UV spectra are obtained for seven sub-classes, including mostly beta ketones, where position and type of substitution on benzene rings give rise to differences in UV maxima and relative intensity of the spectral bands. This aspect is key to distinguishing positional isomers that contain differences in R substitution (mono and di) around the benzene ring, which provides complementary information to electron ionization mass spectrometry, where the latter technique cannot distinguish between these types of positional isomers. In addition, it is possible to ascertain the substitution position based on the UV spectra. For ten sets of positional isomers, it was possible to distinguish most of the positional isomers within a set. For ultra-high performance supercritical fluid chromatography (UHPSFC) versus reversed phase ultra-high performance liquid chromatography (UHPLC), there was at least a 10 nm blue shift in UV maximum (shift to shorter wavelengths). This highlights the importance of taking in account the effect of mobile phase on the UV maximum when performing method development in UHPSFC. Copyright © 2017 John Wiley & Sons, Ltd.
本文介绍了二极管阵列紫外（UV）检测在辅助鉴定合成卡西酮（包括不同亚类和位置异构体）方面的应用。对于35种合成卡西酮，获得了七个亚类的独特紫外光谱，其中大多为β-酮类，苯环上取代基的位置和类型导致紫外最大吸收波长及光谱带相对强度存在差异。这一点对于区分苯环周围R取代（单取代和二取代）存在差异的位置异构体至关重要，为电子电离质谱提供了补充信息，因为后者技术无法区分这些类型的位置异构体。此外，还可以根据紫外光谱确定取代位置。对于十组位置异构体，能够区分组内的大多数位置异构体。对于超高效超临界流体色谱（UHPSFC）与反相超高效液相色谱（UHPLC），紫外最大吸收波长至少有10 nm的蓝移（向较短波长移动）。这突出了在UHPSFC方法开发过程中考虑流动相对紫外最大吸收波长影响的重要性。版权所有© 2017约翰威立父子有限公司。
