State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2017 Mar;228:348-354. doi: 10.1016/j.biortech.2016.12.107. Epub 2017 Jan 3.
An ionic liquid of N-methylmorpholine-N-oxide (NMMO) was used to effectively pretreat cassava residues for the efficient enzymatical hydrolysis and cogeneration of fermentative hydrogen and methane. The reducing sugar yield of enzymolysed cassava residues with NMMO pretreatment improved from 36 to 42g/100g cassava residues. Scanning electron microscopy images revealed the formation of deep grooves (∼4μm wide) and numerous pores in the cassava residues pretreated with NMMO. X-ray diffraction patterns showed that the crystallinity coefficient of NMMO-pretreated cassava residues decreased from 40 to 34. Fourier transform infrared spectra indicated that crystal cellulose I was partially transformed to amorphous cellulose II in the NMMO-pretreated cassava residues. This transformation resulted in a reduced crystallinity index from 0.85 to 0.77. Hydrogen yield from the enzymolysed cassava residues pretreated with NMMO increased from 92.3 to 126mL/gTVS, and the sequential methane yield correspondingly increased from 79.4 to 101.6mL/g TVS.
离子液体 N-甲基吗啉-N-氧化物（NMMO）可有效预处理木薯渣，从而实现木薯渣的高效酶解和发酵氢与甲烷的联产。经 NMMO 预处理的木薯渣的还原糖得率从 36g/100g 木薯渣提高到 42g/100g 木薯渣。扫描电子显微镜图像显示，经 NMMO 预处理的木薯渣形成了深槽（~4μm 宽）和许多孔。X 射线衍射图谱表明，NMMO 预处理木薯渣的结晶度系数从 40 降低到 34。傅里叶变换红外光谱表明，晶态纤维素 I 部分转化为 NMMO 预处理木薯渣中的无定形纤维素 II。这种转化导致结晶度指数从 0.85 降低到 0.77。经 NMMO 预处理的酶解木薯渣的产氢量从 92.3mL/gTVS 增加到 126mL/gTVS，随后的甲烷产量相应从 79.4mL/gTVS 增加到 101.6mL/gTVS。
