De Dobbeleer G, De Graef C, M'Poudi E, Gourdain J M, Heenen M
Department of Dermatology, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
J Am Acad Dermatol. 1989 Nov;21(5 Pt 1):961-5. doi: 10.1016/s0190-9622(89)70283-8.
We report the in vitro reproduction of the classic histologic and ultrastructural features of familial benign chronic pemphigus (FBCP) by seeding suspensions of lesional keratinocytes onto healthy heterologous dead deepidermized dermis (DED). With the use of normal keratinocytes, control cultures showed a well-differentiated epidermis, with keratohyaline granules and lamellar bodies. To the best of our knowledge this is the first successful culture of FBCP with the use of dispersed lesional keratinocytes. The results suggest that in FBCP the epidermis is the site of the defect leading to acantholysis, without any dermal contribution.
我们报告了通过将病变角质形成细胞悬液接种到健康的异源死亡去表皮真皮（DED）上，在体外重现家族性良性慢性天疱疮（FBCP）的经典组织学和超微结构特征。使用正常角质形成细胞的对照培养显示出分化良好的表皮，有透明角质颗粒和板层小体。据我们所知，这是首次使用分散的病变角质形成细胞成功培养FBCP。结果表明，在FBCP中，表皮是导致棘层松解的缺陷部位，真皮没有任何作用。
