多重聚合酶链反应作为一种快速灵敏的方法用于鉴定清真肉类产品中的肉类种类。
Multiplex-PCR As a Rapid and Sensitive Method for Identification of Meat Species in Halal-Meat Products.
作者信息
Alikord Mahsa, Keramat Javad, Kadivar Mahdi, Momtaz Hassan, Eshtiaghi Mohammad N, Homayouni-Rad Aziz
机构信息
Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz. Iran.
Department of Food Science and Technology, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Isfahan. Iran.
出版信息
Recent Pat Food Nutr Agric. 2017;8(3):175-182. doi: 10.2174/2212798409666170113151213.
BACKGROUND
Species identification and authentication in meat products are important subjects for ensuring the health of consumers. The multiplex-PCR amplification and species- specific primer set were used for the identification of horse, donkey, pig and other ruminants in raw and processed meat products.
METHODS
Oligonucleotid primers were designed and patented for amplification of species-specific mitochondrial DNA sequences of each species and samples were prepared from binary meat mixtures.
RESULTS
The results showed that meat species were accurately determined in all combinations by multiplex-PCR, and the sensitivity of this method was 0.001 ng, rendering this technique open to and suitable for use in industrial meat products. It is concluded that more fraud is seen in lower percentage industrial meat products than in higher percentage ones. There was also more fraud found in processed products than in raw ones.
CONCLUSION
This rapid and useful test is recommended for quality control firms for applying more rigorous controls over industrial meat products, for the benefit of target consumers.
背景
肉类产品中的物种鉴定和认证是确保消费者健康的重要课题。多重聚合酶链反应（multiplex-PCR）扩增和物种特异性引物组被用于生肉和加工肉类产品中马、驴、猪及其他反刍动物的鉴定。
方法
设计并获得专利的寡核苷酸引物用于扩增各物种的物种特异性线粒体DNA序列，样本取自二元肉类混合物。
结果
结果表明，通过多重聚合酶链反应可准确测定所有组合中的肉类物种，该方法的灵敏度为0.001纳克，使该技术适用于工业肉类产品。得出的结论是，工业肉类产品中低比例产品的欺诈行为比高比例产品更多。加工产品中的欺诈行为也比生肉产品更多。
结论
建议质量控制公司采用这种快速且有用的检测方法，以便对工业肉类产品实施更严格的控制，从而造福目标消费者。
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