Pourakbari Babak, Mahmoudi Shima, Habibi Reza, Ashtiani Mohammad Taghi Haghi, Sadeghi Reihaneh Hosseinpour, Khodabandeh Mahmoud, Mamishi Setareh
Pediatric Infectious Disease Research Center, Tehran University of Medical Science, Tehran, Iran.
Department of Infectious Diseases, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Infect Disord Drug Targets. 2018;18(2):129-135. doi: 10.2174/1871526517666170113144541.
Acinetobacter baumannii has emerged as an important nosocomial pathogen in the past decades. Due to the prevalence of A. baumannii across the world, suitable typing methods to investigate the epidemiological distribution of the organism have been developed. The aim of this study was to investigate the epidemiological and molecular diversity of A. baumannii strains isolated from nosocomial infections of hospitalized children in Children Medical Center Hospital (CMC), an Iranian referral hospital, in Tehran, Iran.
A total of 27 non-duplicate clinical A. baumannii isolates were collected during October 2013 to March 2014 and tested for antimicrobial resistance to several antibiotic agents. The genetic similarity of the strains was investigated by amplification of Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method.
One predominant RAPD profile (type B) was identified in 15 strains (56% of all typed isolates). Other clusters depicted in the dendrogram, namely A, C, and D comprised 6 (22%), 5 (19%) and 1 (3%) isolates, respectively. All A. baumannii strains were resistant to all antibiotics except colistin.
This study highlights the clonal spread of multidrug-resistant A. baumannii in our hospital. Therefore, the factors responsible for dissemination of such isolates need to be identified, controlled, and prevented to avoid major outbreaks.
在过去几十年中，鲍曼不动杆菌已成为一种重要的医院病原体。由于鲍曼不动杆菌在全球范围内普遍存在，已开发出合适的分型方法来研究该菌的流行病学分布。本研究旨在调查从伊朗德黑兰一家转诊医院——儿童医学中心医院（CMC）住院儿童医院感染中分离出的鲍曼不动杆菌菌株的流行病学和分子多样性。
在2013年10月至2014年3月期间共收集了27株非重复的临床鲍曼不动杆菌分离株，并对其进行了几种抗生素的药敏试验。通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）方法扩增来研究菌株的遗传相似性。
在15株菌株（占所有分型分离株的56%）中鉴定出一种主要的RAPD图谱（B型）。树形图中描绘的其他聚类，即A、C和D分别包含6株（22%）、5株（19%）和1株（3%）分离株。除黏菌素外，所有鲍曼不动杆菌菌株对所有抗生素均耐药。
本研究突出了多重耐药鲍曼不动杆菌在我院的克隆传播。因此，需要识别、控制和预防导致此类分离株传播的因素，以避免重大疫情爆发。
