Sampaio Wlaisa V, Oliveira Karol G, Leão Danuza L, Caldas-Bussiere Maria C, Queiroz Helder L, Paim Fernanda P, Santos Regiane R, Domingues Sheyla F S
Laboratory of Wild Animal Biology and Medicine,Faculty of Veterinary Medicine,Federal University of Pará,Castanhal,Pará,Brazil.
National Primate Center,Ananindeua,Pará,Brazil.
Zygote. 2017 Apr;25(2):141-148. doi: 10.1017/S0967199416000411. Epub 2017 Jan 16.
Sperm morphometry can be applied to identify different animal groups and species and to evaluate sperm quality. Furthermore, knowledge on species-specific differences will help to enhance biological information, as well as to develop efficient reproductive technologies. The aims in the present study were to describe sperm morphometry from the recently characterized species S. collinsi and S. vanzolinii, to verify if the morphometric sperm patterns are similar or different between both species, and to determine if the sperm morphometry is affected by the levels of sperm defects using the S. collinsi as a model. Semen was collected from S. collinsi (n = 10) and S. vanzolinii (n = 2) monkeys, and sperm was submitted to morphological analysis. From the 10 samples from S. collinsi, five presented sperm of poor quality and two subgroups were formed for this species, i.e. high and poor quality sperm. Data on sperm motility and vigour were analysed, as well morphometric parameters on sperm head and tail. It was observed the normal morphometry was correlated with high quality sperm. Poor quality sperm presented smaller and 7% more ellipticity in their head, when compared with high quality sperm. Sperm from S. vanzolinii presented larger head than those from S. collinsi, but tail lengths were similar. Sperm morphometry can be used as a complementary tool to predict sperm motility and vigour for the S. collinsi species, and S. collinsi appear as a suitable model for S. vanzolinii.
精子形态测量学可用于识别不同的动物群体和物种，并评估精子质量。此外，了解物种特异性差异将有助于增加生物学信息，以及开发高效的生殖技术。本研究的目的是描述最近鉴定出的科林斯氏跗猴和凡佐利氏跗猴的精子形态测量学特征，验证这两个物种之间的精子形态模式是相似还是不同，并以科林斯氏跗猴为模型，确定精子形态测量学是否受精子缺陷水平的影响。从科林斯氏跗猴（n = 10）和凡佐利氏跗猴（n = 2）采集精液，并对精子进行形态学分析。从科林斯氏跗猴的10个样本中，有5个样本的精子质量较差，该物种形成了两个亚组，即高质量精子和低质量精子。分析了精子活力和活力数据，以及精子头部和尾部的形态测量参数。观察到正常的形态测量学与高质量精子相关。与高质量精子相比，低质量精子的头部更小，椭圆率高7%。凡佐利氏跗猴的精子头部比科林斯氏跗猴的精子头部大，但尾部长度相似。精子形态测量学可作为预测科林斯氏跗猴精子活力和活力的补充工具，并且科林斯氏跗猴似乎是凡佐利氏跗猴的合适模型。
