Li Wen-Hong
Departments of Cell Biology and of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9039, United States.
Cell Calcium. 2017 Jun;64:65-71. doi: 10.1016/j.ceca.2017.01.002. Epub 2017 Jan 9.
Regulated secretion is a fundamental cellular process that serves diverse functions in neurobiology, endocrinology, immunology, and numerous other aspects of animal physiology. In response to environmental or biological cues, cells release contents of secretory granules into an extracellular medium to communicate with or impact neighboring or distant cells through paracrine or endocrine signaling. To investigate mechanisms governing stimulus-secretion coupling, to better understand how cells maintain or regulate their secretory activity, and to characterize secretion defects in human diseases, probes for tracking various exocytotic events at the cellular or sub-cellular level have been developed over the years. This review summarizes different strategies and recent progress in developing optical probes for monitoring regulated secretion in mammalian cells.
调节性分泌是一种基本的细胞过程，在神经生物学、内分泌学、免疫学以及动物生理学的许多其他方面发挥着多种功能。响应环境或生物信号，细胞将分泌颗粒的内容物释放到细胞外介质中，通过旁分泌或内分泌信号与邻近或远处的细胞进行通信或产生影响。为了研究刺激-分泌偶联的机制，更好地理解细胞如何维持或调节其分泌活性，以及表征人类疾病中的分泌缺陷，多年来已经开发出了用于在细胞或亚细胞水平追踪各种胞吐事件的探针。本综述总结了开发用于监测哺乳动物细胞中调节性分泌的光学探针的不同策略和最新进展。
