在鸡胚模型中通过PCR特征标签诱变鉴定减毒鸡白痢沙门氏菌突变体及相应基因。
Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model.
作者信息
Geng Shizhong, Tian Qin, Guo Rongxian, Jiao Yang, Barrow Paul, Yin Chao, Wang Yaonan, Geng Haopeng, Pan Zhiming, Jiao Xinan
机构信息
Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, UK.
出版信息
Microb Pathog. 2017 Mar;104:146-150. doi: 10.1016/j.micpath.2017.01.014. Epub 2017 Jan 12.
A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation.
禽特异性病原菌鸡白痢沙门氏菌的一个关键特征是其通过卵垂直传播给后代。在本研究中，PCR 特征标签诱变鉴定出鸡白痢沙门氏菌菌株的九个基因，这些基因有助于其在孵化过程中在鸡胚中存活。这些基因参与侵袭、细胞分裂、代谢和细菌防御。所有九个突变菌株在体内和体外的竞争指数以及对鸡胚的毒力评估证实了它们的减毒。
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