HydroLink凝胶电泳（HLGE）。三、双链DNA的高DNA上样量及回收。

HydroLink gel electrophoresis (HLGE). III. High DNA loading capacity and recovery of dsDNA.

作者信息

Smith C L, Ewing C M, Mellon M T, Shorr R G, Jain T

机构信息

Research and Development, AT Biochem, Inc., Malvern, PA 19355.

出版信息

J Biochem Biophys Methods. 1989 Jul;19(1):65-73. doi: 10.1016/0165-022x(89)90051-1.

DOI:10.1016/0165-022x(89)90051-1

PMID:2809068

Abstract

Novel polymers have been prepared for high performance electrophoretic separations of double-stranded DNA (dsDNA). These materials are part of a family of HydroLink high performance electrophoresis polymers. A comparison of the resolving capabilities of dsDNA HydroLink gels to agarose and polyacrylamide separations has been described in an accompanying paper. In this study, we demonstrate that dsDNA HydroLink gels possess ten times the loading capacity of comparable polyacrylamide or agarose gels without compromise to resolution or biological integrity of the separated DNA. A simplified procedure for recovery of separated components is also described.

摘要

已制备出用于双链DNA（dsDNA）高效电泳分离的新型聚合物。这些材料是HydroLink高性能电泳聚合物家族的一部分。一篇相关论文中描述了dsDNA HydroLink凝胶与琼脂糖和聚丙烯酰胺分离的分辨能力比较。在本研究中，我们证明dsDNA HydroLink凝胶的上样量是可比的聚丙烯酰胺或琼脂糖凝胶的十倍，同时不影响分离DNA的分辨率或生物学完整性。还描述了一种回收分离组分的简化方法。