Wahl M, Iwarson S, Snoy P, Gerety R J
Department of Infectious Diseases, University of Göteborg, Ostra Hospital, Sweden.
J Hepatol. 1989 Sep;9(2):198-203. doi: 10.1016/0168-8278(89)90051-2.
To study experimentally the protective effect of post-exposure prophylaxis against hepatitis B (HB), a special preparation of hepatitis B immune globulin (HBIG) was injected intravenously (i.v.) into three chimpanzees simultaneously with, or at different time intervals after, intravenous injection of a titred inoculum of hepatitis B virus (HBV). The HBIG was given either simultaneously with the HBV inoculum, at 4 hours after, or at both 4 hours and 4 weeks after the HBV injection. A fourth chimpanzee received a standard preparation of HBIG intramuscularly (i.m.) at both 4 hours and 4 weeks after receiving the HBV injection. A fifth animal received HBIG i.v. 4 hours after the HBV inoculum and at the same time received its first of three HB vaccine injections. All chimpanzees were followed for 1 year. The animals which received HBIG simultaneously with HBV or received HBIG plus vaccine had no serological or biochemical sign of HB during follow-up. The three animals which received HBIG after HBV inoculation all developed HBs-antigenemia and serum aminotransferase (ALT) elevations. HBsAg did however appear in serum several weeks later than expected for the HBV inoculum used. Post-exposure prophylaxis with HBIG did protect the HBV-exposed chimpanzees, only if HBIG was combined with HB-vaccination or if HBIG was given simultaneously with the HBV inoculum.
为了通过实验研究暴露后预防对乙型肝炎（HB）的保护作用，将一种特殊制备的乙型肝炎免疫球蛋白（HBIG）静脉内（i.v.）注射到三只黑猩猩体内，注射时间与静脉注射已滴定的乙型肝炎病毒（HBV）接种物的时间相同，或在不同时间间隔之后。HBIG在接种HBV接种物的同时、接种后4小时、或在接种HBV后4小时和4周时给予。第四只黑猩猩在接种HBV后4小时和4周时均接受标准制剂的HBIG肌肉内（i.m.）注射。第五只动物在接种HBV接种物后4小时接受HBIG静脉内注射，同时接受三次乙肝疫苗注射中的第一次。所有黑猩猩均随访1年。在随访期间，与HBV同时接受HBIG或接受HBIG加疫苗的动物没有HB的血清学或生化迹象。在接种HBV后接受HBIG的三只动物均出现HBs抗原血症和血清转氨酶（ALT）升高。然而，HBsAg出现在血清中的时间比所用HBV接种物预期的时间晚几周。只有当HBIG与乙肝疫苗联合使用或与HBV接种物同时给予时，用HBIG进行暴露后预防才能保护暴露于HBV的黑猩猩。
