El Aamri Fatima, Guillén José Ángel, Padilla Daniel, Bravo Jimena, Vega Belinda, Acosta Félix, Real Fernando
Institut National de Recherche Halieutique (INRH), M'diq, Morocco.
Unidad Asociada de Biomedicina IIBM CSIC, University of Las Palmas de Gran Canaria, Las Palmas, Spain.
Vet Res Commun. 2017 Jun;41(2):85-95. doi: 10.1007/s11259-017-9675-z. Epub 2017 Jan 14.
The surface-associated proteins play a key role in bacterial physiology and pathogenesis, and are the major targets in the development of new vaccines. These proteins contribute to the adaptation of bacteria to different hosts and environments. To study differences at the genomic level, we first sequenced the whole genome of Streptococcus iniae from fish (IUSA-1 strain) and compared it to Streptococcus iniae from human (9117 strain), revealing a high similitude between both strains. To gain further insights into host- and environment-specific differences, we then studied proteins in silico and by High Performance Liquid Chromatography. This approach successfully identified 54 secreted and surface proteins, including several proteins involved in cell wall synthesis and transport of solutes, as well as proteins with yet unknown function. These proteins highlight as interesting targets for further investigation in the interaction between Streptococcus iniae and its environment. Results reported in this study have shown a first analysis about the predicted and experimental associated proteins of Streptococcus iniae isolated from two different hosts: human and fish.
表面相关蛋白在细菌生理学和发病机制中起关键作用，并且是新型疫苗研发的主要靶点。这些蛋白有助于细菌适应不同的宿主和环境。为了在基因组水平上研究差异，我们首先对来自鱼类的海豚链球菌（IUSA-1菌株）的全基因组进行了测序，并将其与来自人类的海豚链球菌（9117菌株）进行比较，结果显示这两种菌株高度相似。为了进一步深入了解宿主和环境特异性差异，我们随后通过计算机模拟和高效液相色谱法研究了蛋白质。这种方法成功鉴定出54种分泌蛋白和表面蛋白，包括几种参与细胞壁合成和溶质转运的蛋白，以及功能未知的蛋白。这些蛋白凸显出作为进一步研究海豚链球菌与其环境之间相互作用的有趣靶点。本研究报告的结果首次分析了从两种不同宿主（人类和鱼类）分离出的海豚链球菌的预测和实验相关蛋白。
