利用相互作用蛋白-肽阵列筛选技术解析蛋白质相互作用网络

Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks.

作者信息

Liu Huadong, Voss Courtney, Li Shawn S C

机构信息

Department of Biochemistry, Siebens-Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.

出版信息

Methods Mol Biol. 2017;1555:429-436. doi: 10.1007/978-1-4939-6762-9_25.

DOI:10.1007/978-1-4939-6762-9_25

PMID:28092048

Abstract

Protein-protein interactions (PPIs) play a central role in almost all cellular processes. Recent technological advances have enabled the elucidation of an incredibly complex PPI network within the cell. However, protein interactions driven by posttranslational modifications (PTMs) such as phosphorylation, which comprises a significant part of the PPI network, have proven difficult to decipher systematically. Herein, we describe a reciprocal protein-peptide array strategy to uncover PPIs mediated by tyrosine phosphorylation and the Src homology 2 (SH2) domain. This strategy, namely combining peptide and protein domain arrays for PPI mapping, may be applicable for other peptide-binding modules.

摘要

蛋白质-蛋白质相互作用（PPIs）在几乎所有细胞过程中都起着核心作用。最近的技术进步使得人们能够阐明细胞内极其复杂的PPI网络。然而，由翻译后修饰（PTMs）如磷酸化驱动的蛋白质相互作用，虽然构成了PPI网络的重要部分，但已证明难以系统地破译。在此，我们描述了一种相互作用的蛋白质-肽阵列策略，以揭示由酪氨酸磷酸化和Src同源2（SH2）结构域介导的PPIs。这种策略，即结合肽和蛋白质结构域阵列进行PPI图谱分析，可能适用于其他肽结合模块。