一种用于同时分析人单核细胞表面抗原和DNA的流式细胞术。
A flow cytometric technique for simultaneous analysis of human mononuclear cell surface antigens and DNA.
作者信息
Rigg K M, Shenton B K, Murray I A, Givan A L, Taylor R M, Lennard T W
机构信息
Department of Surgery, Medical School, Newcastle upon Tyne, U.K.
出版信息
J Immunol Methods. 1989 Oct 24;123(2):177-84. doi: 10.1016/0022-1759(89)90221-4.
A method for dual staining of mononuclear cells for lymphocyte phenotypic markers and DNA is described. The cells were stained with fluorescein-conjugated monoclonal antibodies and then rendered permeable to propidium iodide using saponin. Propidium iodide stains DNA and, using flow cytometry, cell cycle analysis of individual lymphocyte subpopulations can be determined. Saponin acts within 1 min, preserves expression of surface antigens and is effective at all concentrations from 0.001% to 1%. This technique is simple, rapid and gives reproducible results.
描述了一种对单核细胞进行淋巴细胞表型标志物和DNA双重染色的方法。细胞先用荧光素偶联的单克隆抗体染色，然后用皂素使细胞对碘化丙啶具有通透性。碘化丙啶可对DNA进行染色，通过流式细胞术可确定各个淋巴细胞亚群的细胞周期分析。皂素在1分钟内起作用，能保留表面抗原的表达，在0.001%至1%的所有浓度下均有效。该技术简单、快速且结果可重复。
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