Bekisz J B, zur Nedden D L, Enterline J C, Zoon K C
Division of Cytokine Biology, Center for Biologies Evaluation and Research, Bethesda, MD 20892.
J Interferon Res. 1989 Sep;9 Suppl 1:S1-7.
Some patients treated with interferon (IFN) have developed antibodies (ABs) to that IFN. We examined the incidence of such ABs in hairy cell leukemia (HCL) patients treated with IFN-alpha 2a and IFN-alpha 2b. In the initial enzyme-linked immunosorbent assay (ELISA) assays, the serum samples were tested against their corresponding IFNs. Of 73 evaluable patients treated with IFN-alpha 2b, 6 patients, who tested negative prior to treatment, were positive by ELISA following treatment with IFN. Two of the samples tested positive in the neutralization assay. Regarding the samples from patients treated with IFN-alpha 2a, 2 of the 44 patients were ELISA positive following IFN treatment (while being negative prior to treatment) and 2 others became positive in the neutralization assay following IFN treatment. Five serum samples with neutralizing activity were tested for their ability to bind to each of eight natural IFN-alpha species derived from human lymphoblastoid cells induced with Sendai virus; a different cross-reactivity profile was seen for each sample. In conclusion, a low incidence of neutralizing and nonneutralizing ABs against IFN-alpha 2 was observed in patients with HCL treated with recombinant DNA-derived IFN-alpha 2a or IFN-alpha 2b. Finally, ABs against these IFNs cross-react by ELISA with several naturally occurring IFN-alpha s.
一些接受干扰素（IFN）治疗的患者产生了针对该干扰素的抗体（ABs）。我们检测了接受α-2a干扰素和α-2b干扰素治疗的毛细胞白血病（HCL）患者中此类抗体的发生率。在最初的酶联免疫吸附测定（ELISA）试验中，血清样本针对相应的干扰素进行检测。在73例接受α-2b干扰素治疗的可评估患者中，6例治疗前检测为阴性的患者在接受干扰素治疗后ELISA检测呈阳性。其中2份样本在中和试验中呈阳性。对于接受α-2a干扰素治疗患者的样本，44例患者中有2例在接受干扰素治疗后ELISA呈阳性（治疗前为阴性），另外2例在接受干扰素治疗后的中和试验中呈阳性。对5份具有中和活性的血清样本检测它们与8种源自仙台病毒诱导的人淋巴母细胞的天然α-干扰素结合的能力；每份样本呈现出不同的交叉反应谱。总之，在接受重组DNA衍生的α-2a干扰素或α-2b干扰素治疗的HCL患者中，观察到针对α-2干扰素的中和及非中和抗体的发生率较低。最后，针对这些干扰素的抗体通过ELISA与几种天然存在的α-干扰素发生交叉反应。
