Hetzel Terence, Blaesing Christina, Jaeger Martin, Teutenberg Thorsten, Schmidt Torsten C
Institut für Energie- und Umwelttechnik e. V., IUTA (Institute of Energy and Environmental Technology), Bliersheimer Str. 58-60, 47229 Duisburg, Germany; Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, 45141 Essen, Germany.
Institut für Energie- und Umwelttechnik e. V., IUTA (Institute of Energy and Environmental Technology), Bliersheimer Str. 58-60, 47229 Duisburg, Germany.
J Chromatogr A. 2017 Feb 17;1485:62-69. doi: 10.1016/j.chroma.2017.01.018. Epub 2017 Jan 8.
The performance of micro-liquid chromatography columns with an inner diameter of 0.3mm was investigated on a dedicated micro-LC system for gradient elution. Core-shell as well as fully porous particle packed columns were compared on the basis of peak capacity and gradient kinetic plot limits. The results for peak capacity showed the superior performance of columns packed with sub-2μm fully porous particles compared to 3.0μm fully porous and 2.7μm core-shell particles within a range of different gradient time to column void time ratios. For ultra-fast chromatography a maximum peak capacity of 16 can be obtained using a 30s gradient for the sub-2μm fully porous particle packed column. A maximum peak capacity of 121 can be achieved using a 5min gradient. In addition, the influence of an alternative detector cell on the basis of optical waveguide technology and contributing less to system variance was investigated showing an increased peak capacity for all applied gradient time/column void time ratios. Finally, the influence of pressure was evaluated indicating increased peak capacity for maximum performance whereas a limited benefit for ultra-fast chromatography with gradient times below 30s was observed.
在一台用于梯度洗脱的专用微液相色谱系统上，对内径为0.3mm的微液相色谱柱的性能进行了研究。基于峰容量和梯度动力学曲线极限，对核壳型以及全多孔颗粒填充柱进行了比较。峰容量的结果表明，在不同梯度时间与柱死时间比的范围内，填充2μm以下全多孔颗粒的色谱柱性能优于填充3.0μm全多孔颗粒和2.7μm核壳型颗粒的色谱柱。对于超快速色谱，使用30s梯度对填充2μm以下全多孔颗粒的色谱柱可获得最大峰容量16。使用5min梯度可实现最大峰容量121。此外，还研究了基于光波导技术且对系统方差贡献较小的替代检测池的影响，结果表明在所有应用的梯度时间/柱死时间比下峰容量均有所增加。最后，评估了压力的影响，结果表明在最大性能下峰容量增加，而对于梯度时间低于30s的超快速色谱，观察到的益处有限。
