Ikeda K, Oda Y, Tomita K, Nomura S, Nakanishi I
J Electron Microsc (Tokyo). 1989;38(4):230-4.
The purpose of the present study is to examine whether isolated Schwann cells that are not in contact with axons can synthesize the basement membrane in vivo. Schwann cells obtained from sciatic nerve of neonate Wistar rats were prepared in culture, harvested, and mixed with collagen matrix. The silicone tube filled with the collagen matrix containing Schwann cells was closed by a Millipore membrane at both ends to isolate the interior from the outer environment and was implanted in a gap between proximal and distal stumps of transected sciatic nerve of an adult Wistar rat. Ten days later, the implanted silicone tube was prepared for electron microscopic observations. Schwann cells in the tube were elongated longitudinally and lined up parallel to the proximo-distal direction. No regenerating axons penetrated the Millipore membrane into the tube. The basement membrane showing almost normal structure was produced on the surface of the Schwann cells. While the silicone tube was placed side by side to the intact sciatic nerve, Schwann cells in the tube were scattered and no basement membrane was observed. These results suggest that some humoral factors might be released from the transected nerve stumps, which may be responsible for the regular arrangement and the basement membrane formation by Schwann cells without any contact with axons.
本研究的目的是检测未与轴突接触的孤立雪旺细胞在体内是否能合成基底膜。从新生Wistar大鼠坐骨神经获取雪旺细胞，进行培养、收获后与胶原基质混合。将装有含雪旺细胞胶原基质的硅胶管两端用微孔膜封闭，使内部与外部环境隔离，然后植入成年Wistar大鼠坐骨神经横断的近端和远端残端之间的间隙中。十天后，对植入的硅胶管进行电子显微镜观察。管内的雪旺细胞纵向伸长，并沿近端至远端方向排列。没有再生轴突穿过微孔膜进入管内。在雪旺细胞表面产生了结构几乎正常的基底膜。当硅胶管与完整的坐骨神经并排放置时，管内的雪旺细胞分散，未观察到基底膜。这些结果表明，横断的神经残端可能释放了一些体液因子，这可能是雪旺细胞在未与轴突接触的情况下进行规则排列和形成基底膜的原因。
