de Menezes Thais Almeida, Bispo Aline Simoes da Rocha, Koblitz Maria Gabriela Bello, Vandenberghe Luciana Porto de Souza, Kamida Helio Mitoshi, Goes-Neto Aristoteles
Department of Biological Sciences, State University of Feira de Santana (UEFS), Novo Horizonte, Feira de Santana, BA, Brazil.
Department of Food Technology, Federal University of the State of Rio de Janeiro (UNIRIO), Rio de Janeiro, RJ, Brazil.
Int J Med Mushrooms. 2016;18(12):1141-1149. doi: 10.1615/IntJMedMushrooms.v18.i12.90.
Ganoderma lucidum is a medicinal mushroom with different forms of bioactivity that has been used in popular medicine for centuries. This study aimed to test the application of agricultural wastes (fruit shells, leaves, and bracts) from the endemic Brazilian palm tree Syagrus coronata (licuri) as substrates for the production of G. lucidum basidiomata and ligninolytic enzymes via solid-state fermentation. The best culture conditions were the same for all substrates (pH 6.5, carbon-to-nitrogen ratio = 40, and temperature 30°C) and were established from preliminary assays. The yield was not significantly different for bracts (33.53 g/kg) and leaves (37.48 g/kg), nor for the biological efficiency in these same substrates: bracts, 3.35%; leaves, 3.75%. The highest laccase (13.80 U/L) and manganese peroxidase (14.92 U/L) activities were achieved after 14 and 28 days of incubation, respectively, using bracts as the substrate. Licuri residues are then potential substrates to be used in the bioconversion process for mycelia, basidiomata, and ligninolytic enzyme production by G. lucidum.
灵芝是一种具有多种生物活性形式的药用真菌，几个世纪以来一直在民间医学中使用。本研究旨在测试来自巴西本土棕榈树Syagrus coronata（利库里棕榈）的农业废弃物（果壳、树叶和苞片）作为固态发酵生产灵芝子实体和木质素分解酶的底物的应用。所有底物的最佳培养条件相同（pH 6.5、碳氮比 = 40、温度30°C），并通过初步试验确定。苞片（33.53 g/kg）和树叶（37.48 g/kg）的产量以及这些相同底物中的生物学效率没有显著差异：苞片为3.35%；树叶为3.75%。以苞片为底物培养14天和28天后，分别获得了最高的漆酶（13.80 U/L）和锰过氧化物酶（14.92 U/L）活性。因此，利库里棕榈残渣是用于灵芝菌丝体、子实体和木质素分解酶生物转化过程的潜在底物。
