Bornfeldt K E, Arnqvist H J, Enberg B, Mathews L S, Norstedt G
Department of Pharmacology, Faculty of Health Sciences, Linköping University, Sweden.
J Endocrinol. 1989 Sep;122(3):651-6. doi: 10.1677/joe.0.1220651.
Insulin-like growth factor-I (IGF-I) mRNA and GH receptor mRNA levels were analysed in different tissues from rats made diabetic with streptozotocin, fasted rats and rats fed with a protein-reduced diet. Diabetes decreased IGF-I mRNA levels in liver, heart, diaphragm, kidney and aorta, but not in brain. GH receptor mRNA levels were decreased in heart and diaphragm, but not in liver and kidney. Fasting decreased IGF-I mRNA in all tissues studied except brain, and decreased GH receptor mRNA in liver, heart and diaphragm, but not in kidney. A protein-reduced diet decreased hepatic IGF-I mRNA levels but did not significantly affect other tissues, while GH receptor mRNA levels were reduced in liver and diaphragm. In conclusion, both diabetes and limited nutrition affected IGF-I and GH receptor mRNA in different tissues, but the two mRNAs were not co-ordinately regulated in all tissues studied. While reduced GH receptor gene expression may thus be responsible for decreased IGF-I gene expression in some states and tissues, additional regulatory mechanisms may be of importance.
对用链脲佐菌素诱导糖尿病的大鼠、禁食大鼠以及食用低蛋白饮食的大鼠不同组织中的胰岛素样生长因子-I（IGF-I）mRNA和生长激素受体mRNA水平进行了分析。糖尿病使肝脏、心脏、膈肌、肾脏和主动脉中的IGF-I mRNA水平降低，但大脑中的未降低。心脏和膈肌中的生长激素受体mRNA水平降低，但肝脏和肾脏中的未降低。禁食使除大脑外所有研究组织中的IGF-I mRNA降低，使肝脏、心脏和膈肌中的生长激素受体mRNA降低，但肾脏中的未降低。低蛋白饮食使肝脏IGF-I mRNA水平降低，但对其他组织无显著影响，而肝脏和膈肌中的生长激素受体mRNA水平降低。总之，糖尿病和营养受限均影响不同组织中的IGF-I和生长激素受体mRNA，但在所研究的所有组织中这两种mRNA并非协同调节。因此，虽然生长激素受体基因表达降低可能在某些状态和组织中导致IGF-I基因表达降低，但其他调节机制可能也很重要。
