Goodrowe K L, Miller A M, Wildt D E
National Zoological Park, Smithsonian Institution, Washington, DC 20008.
J Exp Zool. 1989 Oct;252(1):89-95. doi: 10.1002/jez.1402520112.
Based on techniques developed for the domestic cat, in vitro fertilization (IVF) studies were conducted in the taxonomically related leopard cat (Felis bengalensis). Adult females received pregnant mares' serum gonadotropin (PMSG) followed 80 or 84 h later by human chorionic gonadotropin (hCG) on two to four occasions over a 40-day to 27-month interval. Oocytes were collected laparoscopically from ovarian follicles 25-27 h after hCG and co-cultured with processed, homologous spermatozoa (37 degrees C, 5% CO2 in air, humidified atmosphere) for 30-36 h. There was no apparent ovarian refractoriness to repeated treatments with exogenous gonadotropins. Overall, the mean number of mature follicles present and the total number of oocytes and proportion of immature oocytes collected did not differ (P greater than 0.05) between the 80 h (4.9 +/- 0.9; 4.7 +/- 1.2; 14.9%, respectively) and 84 h (5.6 +/- 1.4; 5.4 +/- 1.7; 22.2%, respectively) gonadotropin interval groups. However, the proportion of mature leopard cat oocytes fertilized in vitro, as determined by embryonic cleavage, was increased (P less than 0.005) by extending the interval between PMSG and hCG from 80 (17.5%) to 84 (52.4%) h. These data 1) demonstrate that, compared to the domestic cat, the ovaries of the leopard cat are less responsive to a given PMSG/hCG treatment; 2) indicate that leopard cat follicular oocytes can be recovered readily by laparoscopy and are capable of becoming fertilized in vitro; and 3) suggest that IVF may be a viable approach for producing embryos and perhaps enhancing captive propagation of rare Felidae.
基于为家猫开发的技术，在分类学上相关的豹猫（豹猫属）中进行了体外受精（IVF）研究。成年雌性接受孕马血清促性腺激素（PMSG），在40天至27个月的间隔内，分两到四次，在80或84小时后注射人绒毛膜促性腺激素（hCG）。在hCG注射后25 - 27小时，通过腹腔镜从卵巢卵泡中收集卵母细胞，并与处理过的同源精子（37摄氏度，空气中5%二氧化碳，湿润环境）共同培养30 - 36小时。对于外源性促性腺激素的重复治疗，卵巢没有明显的不应性。总体而言，在80小时（分别为4.9±0.9；4.7±1.2；14.9%）和84小时（分别为5.6±1.4；5.4±1.7；22.2%）促性腺激素间隔组之间，成熟卵泡的平均数量、收集的卵母细胞总数以及未成熟卵母细胞的比例没有差异（P大于0.05）。然而，通过胚胎分裂确定的体外受精的豹猫成熟卵母细胞比例，通过将PMSG和hCG之间的间隔从80小时（17.5%）延长到84小时（52.4%）而增加（P小于0.005）。这些数据1）表明，与家猫相比，豹猫的卵巢对给定的PMSG/hCG治疗反应较小；2）表明通过腹腔镜可以很容易地回收豹猫卵泡卵母细胞，并且它们能够在体外受精；3）表明体外受精可能是生产胚胎以及可能增强珍稀猫科动物圈养繁殖的可行方法。
