Abdulrahman Al Asmari, Faisal Kunnathodi, Meshref Ali Al Amri, Arshaduddin Mohammed
a Scientific Research Center, Medical Services Department , Riyadh , Saudi Arabia.
b Department of Pharmacy and Pharmaceutical Sciences, Medical Services Department , Riyadh , Saudi Arabia.
Neurol Res. 2017 Mar;39(3):264-270. doi: 10.1080/01616412.2016.1275456. Epub 2017 Jan 18.
To study the effect of pretreatment with low doses of vanillin, a flavoring agent used as a food additive, on harmaline-induced tremor in rats.
Sprague Dawley rats (110 ± 5 g) were divided into groups of six animals each. Vanillin (6.25 mg, 12.5 mg, and 25 mg/kg) was administered by gavage to different groups of rats, 30 minutes before the induction of tremor. Harmaline (10 mg/kg, i.p.) was used for the induction of tremor. The latency of onset, duration, tremor intensity, tremor index, and spontaneous locomotor activity were recorded. A separate batch of animals was used for the determination of serotonin (5HT) and 5 hydroxyindole acetic acid (5HIAA) levels in the brain.
Harmaline treatment resulted in characteristic tremor that lasted for more than 2 hours and decreased the locomotor activity of rats. Pre-treatment with vanillin significantly reduced the duration, intensity, and tremor index of harmaline-treated animals. Vanillin treatment also significantly attenuated harmaline-induced decrease in the locomotor activity. An increase in 5HT levels and the changes in 5HIAA/5HT ratio observed in harmaline treated rats were significantly corrected in vanillin pretreated animals.
Vanillin in low doses reduces harmaline-induced tremor in rats, probably through its modulating effect on serotonin levels in the brain. These findings suggest a beneficial effect of vanillin in essential tremor.
研究低剂量香草醛（一种用作食品添加剂的调味剂）预处理对大鼠中哈马灵诱导震颤的影响。
将斯普拉格 - 道利大鼠（110 ± 5 g）分成每组六只动物的组。在诱导震颤前30分钟，通过灌胃向不同组的大鼠给予香草醛（6.25 mg、12.5 mg和25 mg/kg）。使用哈马灵（10 mg/kg，腹腔注射）诱导震颤。记录发作潜伏期、持续时间、震颤强度、震颤指数和自发运动活动。另一批动物用于测定脑中血清素（5HT）和5 - 羟吲哚乙酸（5HIAA）水平。
哈马灵处理导致特征性震颤持续超过2小时，并降低了大鼠的运动活动。香草醛预处理显著降低了哈马灵处理动物的震颤持续时间、强度和震颤指数。香草醛处理还显著减轻了哈马灵诱导的运动活动降低。在香草醛预处理的动物中，哈马灵处理大鼠中观察到的5HT水平升高以及5HIAA/5HT比值变化得到显著纠正。
低剂量香草醛可减轻大鼠中哈马灵诱导的震颤，可能是通过其对脑中血清素水平的调节作用。这些发现表明香草醛对特发性震颤有有益作用。
