Chen Yongxia, Huang Linkai, Yan Haidong, Zhang Xinquan, Xu Bin, Ma Xiao
Animal Science Department, Xichang college, Xichang, 615000 China.
Grassland Science Department, Sichuan Agricultural University, Ya'an, 625014 China.
Hereditas. 2016 Apr 8;153:3. doi: 10.1186/s41065-016-0008-y. eCollection 2016.
is a stoloniferous perennial tropical forage grass with a wide geographic distribution; however, environmental stress has a great influence on its growth. The DREB transcription factor family genes contains candidate genes for improving plant stress tolerance.
From cold-treated plants, a putative gene () was cloned using the RACE-PCR method. was 1296 bp in length and encoded a putative protein 264 amino acid residues long. shared the highest sequence identity with in sorghum. The expression of HcDREB2 was independent of ABA treatment, but inducible by low temperatures as well as drought and high salinity treatments. Yeast one-hybrid assays showed that HcDREB2 directly bound the DRE cis-acting element to transactivate the expression of the downstream reporter genes.
HcDREB2, a stress-inducible but ABA-independent transcription factor gene, can transactivate downstream genes by binding to the DRE cis-element. The current results are a foundation for making use of this stress tolerance gene in future studies.
[植物名称]是一种具有匍匐茎的多年生热带饲草，地理分布广泛；然而，环境胁迫对其生长有很大影响。DREB转录因子家族基因包含提高植物胁迫耐受性的候选基因。
从经冷处理的植株中，采用RACE-PCR方法克隆了一个假定基因（HcDREB2）。HcDREB2长度为1296 bp，编码一个由264个氨基酸残基组成的假定蛋白质。HcDREB2与高粱中的[基因名称]具有最高的序列同一性。HcDREB2的表达不依赖于ABA处理，但可被低温以及干旱和高盐处理诱导。酵母单杂交试验表明，HcDREB2直接结合DRE顺式作用元件以反式激活下游报告基因的表达。
HcDREB2是一种胁迫诱导但不依赖ABA的转录因子基因，可通过与DRE顺式元件结合来反式激活下游基因。目前的结果为在未来[植物名称]研究中利用这种胁迫耐受性基因奠定了基础。
