Odilbekov Firuz, Edin Eva, Garkava-Gustavsson Larisa, Hovmalm Helena Persson, Liljeroth Erland
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, P.O. Box 102, SE-230 53 Alnarp, Sweden.
Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-750 07 Uppsala, Sweden.
Hereditas. 2016 Sep 23;153:10. doi: 10.1186/s41065-016-0014-0. eCollection 2016.
Early blight, caused by the fungus occurs on potato mainly in the south-eastern part of Sweden, but also in other parts of the country. The aim of this study was to investigate the genetic diversity of populations from different potato growing regions in south-eastern Sweden using AFLP marker analysis. In addition, the cultured isolates were examined for substitutions in the gene encoding cytochrome , associated with loss of sensitivity against QoI fungicides.
Nei's gene diversity index for the Swedish populations of revealed a gene diversity of up to 0.20. Also genetic differentiation was observed among populations of from different locations in south-eastern Sweden. The mitochondrial genotype of the isolates of was determined and both known genotypes, GI (genotype 1) and GII (genotype 2), were found among the isolates. The occurrence of the F129L substitution associated with a loss of sensitivity to strobilurins was confirmed among the GII isolates. In vitro conidial germination tests verified that isolates containing the F129L substitution had reduced sensitivity to azoxystrobin and, at a lower extent, to pyraclostrobin.
Genetic diversity was relatively high among isolates of in south-eastern part of Sweden. F129L substitutions, leading to reduced sensitivity to strobilurins, have been established in field populations, which may have implications for the future efficacy of QoI fungicides.
由该真菌引起的早疫病主要发生在瑞典东南部的马铃薯上，但该国其他地区也有发生。本研究的目的是利用AFLP标记分析，调查瑞典东南部不同马铃薯种植区的该菌种群的遗传多样性。此外，对培养的分离株进行检测，以确定编码细胞色素的基因中的替代情况，这与对QoI类杀菌剂敏感性丧失有关。
瑞典该菌种群的Nei基因多样性指数显示基因多样性高达0.20。在瑞典东南部不同地点的该菌种群之间也观察到了遗传分化。确定了该菌分离株的线粒体基因型，在分离株中发现了两种已知基因型，即GI（基因型1）和GII（基因型2）。在GII分离株中证实了与对甲氧基丙烯酸酯类杀菌剂敏感性丧失相关的F129L替代的出现。体外分生孢子萌发试验证实，含有F129L替代的分离株对嘧菌酯的敏感性降低，对吡唑醚菌酯的敏感性在较低程度上也降低。
瑞典东南部该菌分离株的遗传多样性相对较高。导致对甲氧基丙烯酸酯类杀菌剂敏感性降低的F129L替代已在田间种群中出现，这可能对QoI类杀菌剂的未来疗效产生影响。
