Shahabi Sima, Fekrazad Reza, Johari Maryam, Chiniforoush Nasim, Rezaei Yashar
Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran; Departmet of Biomaterials, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran; Laser Research Center in Medical Sciences (LRCMS), AJA University of Medical Sciences, Tehran, Iran.
J Dent Res Dent Clin Dent Prospects. 2016 Fall;10(4):207-212. doi: 10.15171/joddd.2016.033. Epub 2016 Dec 21.
Despite recent advances in dental caries prevention, caries is common and remains a serious health problem. Laser irradiation is one of the most common methods in preventive measures in recent years. Raman spectroscopy technique is utilized to study the microcrystalline structure of dental enamel. In this study, FT-Raman spectroscopy was used to evaluate chemical changes in enamel structure irradiated with Nd:YAG and Er:YAG lasers. We used 15 freshly-extracted, non-carious, human molars that were treated as follows: No treatment was carried out in group A (control group); Group B was irradiated with Er:YAG laser for 10 seconds under air and water spray; and Group C was irradiated with Nd:YAG laser for 10 seconds under air and water spray. After treatment, the samples were analyzed by FT-Raman spectroscopy. The carbonate content evaluation with regard to the integrated area under the curve (1065/960 cm) exhibited a significant reduction in its ratio in groups B and C. The organic content (2935/960 cm) area exhibited a significant decrease after laser irradiation in group B and C. The results showed that the mineral and organic matrices of enamel structure were affected by laser irradiation; therefore, it might be a suitable method for caries prevention.
尽管近年来在龋齿预防方面取得了进展，但龋齿仍然很常见，并且仍然是一个严重的健康问题。激光照射是近年来预防措施中最常用的方法之一。拉曼光谱技术用于研究牙釉质的微晶结构。在本研究中，傅里叶变换拉曼光谱（FT-Raman光谱）被用于评估经钕：钇铝石榴石（Nd:YAG）和铒：钇铝石榴石（Er:YAG）激光照射的牙釉质结构中的化学变化。我们使用了15颗新拔除的、无龋的人类磨牙，并进行如下处理：A组（对照组）不进行处理；B组在空气和水喷雾下用Er:YAG激光照射10秒；C组在空气和水喷雾下用Nd:YAG激光照射10秒。处理后，通过FT-Raman光谱对样品进行分析。关于曲线下积分面积（1065/960 cm）的碳酸盐含量评估显示，B组和C组的该比例显著降低。有机含量（2935/960 cm）面积在B组和C组激光照射后显著减少。结果表明，牙釉质结构的矿物质和有机基质受到激光照射的影响；因此，它可能是一种合适的龋齿预防方法。
