Harada K
Nihon Jibiinkoka Gakkai Kaiho. 1989 Jul;92(7):1049-64. doi: 10.3950/jibiinkoka.92.1049.
The purpose of this study was to quantitatively and qualitatively analyze the age-related histopathological changes of human palatine tonsil. As for specimens of total 118 tonsils, 52 palatine tonsils obtained at autopsy and 66 palatine tonsils obtained by tonsillectomy from patients with the diagnosis of chronic tonsillitis were used. Of these specimens, 71 were from male and 47 were from female, and the age ranged from 6 months to 89 years. The palatine tonsil was routinely sectioned longitudinal at its median, toward the tonsil hilus. 4 microns thick sections were prepared and then stained with hematoxylin-eosin, and according to the elastica-van Gieson staining method. The I-BAS one picture analyzer of ZEISS Co. was used to determine the areas covered with the parenchymal (lymphatic tissue) and extraparenchymal tissue (crypt, fibrous connective tissue and fatty generation) of the palatine tonsil. The results obtained are as follows; 1) The ratio of the parenchymal area to the total area of tonsils, in both specimens obtained at autopsy and tonsillectomy, decreased with age. 2) The average area of lymphoid follicles, in both specimens obtained at autopsy and tonsillectomy, decreased with age. 3) The number of lymphoid follicles per unit area, in both specimens obtained at autopsy and tonsillectomy, did not show any correlation with age. However, the number remarkably decreased in patients with the age of above 70. 4) The lymphocyte infiltration number per unit area of crypt epithelium, in both specimens obtained at autopsy and tonsillectomy, decreased with age. 5) The ratio of crypt area to the total area, in both specimens obtained at autopsy and tonsillectomy, did not show any correlation with age. However, increasing tendency with age was seen. 6) The ratio of fibrous connective tissue area to the total area for, in both specimens obtained at autopsy and tonsillectomy, increased with age. 7) Collagen fibers and elastic fibers, in both specimens obtained at autopsy and tonsillectomy, showed the tendency to proliferate with age. 8) Fatty degeneration was shown to begin in specimens of about 25 years old in age, and subsequently, was seen in most of specimens of above 35 years in age. Fatty degeneration tends to increase with age. The above results indicate that the parenchymal and extraparenchymal tissues of the palatine tonsil undergo both quantitative and qualitative changes with age.
本研究的目的是对人腭扁桃体与年龄相关的组织病理学变化进行定量和定性分析。对于总共118个扁桃体标本，使用了52个尸检时获得的腭扁桃体和66个从诊断为慢性扁桃体炎的患者扁桃体切除术中获得的腭扁桃体。在这些标本中，71个来自男性，47个来自女性，年龄范围从6个月至89岁。腭扁桃体常规在其中线处纵向切开，朝向扁桃体门。制备4微米厚的切片，然后用苏木精-伊红染色，并按照弹性纤维-范吉森染色法染色。使用蔡司公司的I-BAS图像分析仪确定腭扁桃体实质（淋巴组织）和实质外组织（隐窝、纤维结缔组织和脂肪生成）覆盖的面积。获得的结果如下：1）在尸检和扁桃体切除术中获得的标本中，实质面积与扁桃体总面积的比率均随年龄增长而降低。2）在尸检和扁桃体切除术中获得的标本中，淋巴滤泡的平均面积均随年龄增长而减小。3）在尸检和扁桃体切除术中获得的标本中，单位面积的淋巴滤泡数量与年龄无任何相关性。然而，在70岁以上的患者中数量显著减少。4）在尸检和扁桃体切除术中获得的标本中，隐窝上皮单位面积的淋巴细胞浸润数量均随年龄增长而减少。5）在尸检和扁桃体切除术中获得的标本中，隐窝面积与总面积的比率与年龄无任何相关性。然而，随年龄增长有增加趋势。6）在尸检和扁桃体切除术中获得的标本中，纤维结缔组织面积与总面积的比率随年龄增长而增加。7）在尸检和扁桃体切除术中获得的标本中，胶原纤维和弹性纤维均显示出随年龄增长而增殖的趋势。8）脂肪变性在约25岁的标本中开始出现，随后在大多数35岁以上的标本中可见。脂肪变性倾向于随年龄增长而增加。上述结果表明，腭扁桃体的实质和实质外组织随年龄增长发生了定量和定性变化。
