Farrell Kate S, Chapman Phillip L, Sullivan Lauren A
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences.
the Department of Statistics, College of Natural Sciences, Colorado State University, Fort Collins, CO, 80523.
J Vet Emerg Crit Care (San Antonio). 2017 Mar;27(2):206-211. doi: 10.1111/vec.12571. Epub 2017 Jan 18.
To validate a smartphone-based spectrophotometric assay for point-of-care (POC) measurement of hemoglobin (Hgb) concentration against the standard measurement method in dogs without increases in serum lipemia, hemolysis, or bilirubin.
Prospective observational study.
University teaching hospital.
One hundred thirty-nine dogs that had a CBC and corresponding biochemical profile submitted to the clinical pathology laboratory.
None.
Blood tubes submitted for CBC were collected for hemoglobin (Hgb) measurements performed on a POC smartphone device. Each whole blood sample was run on the smartphone in duplicate using 2 strips, for a total of 4 POC Hgb readings per dog. Data collected for each dog included CBC Hgb value, 4 POC Hgb values, and select biochemistry values (bilirubin, lipemia, hemolysis, icterus). A calibration equation was estimated using a weighted linear regression: estimated CBC = (avgPOC-0.4871)/1.0015. For each dog, the percent error was computed between estimated and actual Hgb values; 95% of the percent errors ranged from -13.2% to 20.1%. The standard deviation of percent errors was 7.9% overall. When samples were further divided according to CBC Hgb concentration (low, normal or high), the standard deviation of percent error was 6.7% when Hgb<13 g/dL [130 g/L], 8.9% when Hgb 13-20 g/dL [130-200 g/L], and 6.5% when Hgb>20 g/dL [200 g/L]. The coefficient of variability among the 4 individual POC readings was 3.4%.
A smartphone-based photometric method for measuring Hgb represents a clinically useful POC alternative to a standard laboratory Hgb measurement. There was excellent intrasample reproducibility, and the standard deviation of percent errors was relatively constant across CBC ranges. Additional sampling of patients with a greater range of diseases and biochemical abnormalities that may influence spectrophotometric assays (ie, abnormal bilirubin, icterus, lipemia, hemolysis) is warranted to extend the findings of this study.
验证一种基于智能手机的分光光度法，用于在犬类中进行即时检测（POC）血红蛋白（Hgb）浓度，且不受血清脂血、溶血或胆红素升高的影响，与标准测量方法进行对比。
前瞻性观察研究。
大学教学医院。
139只提交了全血细胞计数（CBC）及相应生化指标至临床病理实验室的犬。
无。
提交用于CBC的血样管用于在即时检测智能手机设备上进行血红蛋白（Hgb）测量。每个全血样本在智能手机上使用2条试纸条重复检测，每只犬共获得4个即时检测Hgb读数。为每只犬收集的数据包括CBC Hgb值、4个即时检测Hgb值以及选定的生化值（胆红素、脂血、溶血、黄疸）。使用加权线性回归估计校准方程：估计的CBC =（平均即时检测值 - 0.4871）/1.0015。计算每只犬估计的和实际的Hgb值之间的误差百分比；95%的误差百分比范围为 - 13.2%至20.1%。总体误差百分比的标准差为7.9%。当根据CBC Hgb浓度（低、正常或高）进一步划分样本时，当Hgb<13 g/dL [130 g/L]时，误差百分比的标准差为6.7%；当Hgb为13 - 20 g/dL [130 - 200 g/L]时，为8.9%；当Hgb>20 g/dL [200 g/L]时，为6.5%。4个单独的即时检测读数之间的变异系数为3.4%。
一种基于智能手机的测量Hgb的光度法是标准实验室Hgb测量的一种临床上有用的即时检测替代方法。样本内具有出色的可重复性，且误差百分比的标准差在CBC范围内相对恒定。有必要对更多患有可能影响分光光度法检测的疾病和生化异常（即异常胆红素、黄疸、脂血、溶血）的患者进行额外采样，以扩展本研究的结果。
