白藜芦醇与古古甾酮联合治疗对小鼠轻度急性胰腺炎的影响。

Effect of Combinatory Treatment With Resveratrol and Guggulsterone on Mild Acute Pancreatitis in Mice.

作者信息

Kim Nayoung, Park Jin Myung, Lee Sang Hyub, Kim Bo Hye, Son Jun Hyuk, Ryu Ji Kon, Kim Yong-Tae, Lee Woochang

机构信息

From the *Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine; †Department of Internal Medicine and Liver Research Institute, Seoul National University Hospital, Seoul National University College of Medicine, Jongno-gu, Seoul; ‡Department of Internal Medicine, Kangwon National University School of Medicine, Kangwon National University Hospital, Chuncheon; and §Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

出版信息

Pancreas. 2017 Mar;46(3):366-371. doi: 10.1097/MPA.0000000000000763.

DOI:10.1097/MPA.0000000000000763

PMID:28099255

Abstract

OBJECTIVES

This study was conducted to assess the preventive/therapeutic effects of combined administration of resveratrol and guggulsterone on cerulein-induced acute pancreatitis in mice.

METHODS

Acute pancreatitis was induced by intraperitoneal injection of cerulein in mice. Serum amylase assay and histology were performed to measure the severity of pancreatitis. Western blotting and multiplex cytokine/chemokine analysis were conducted to understand the action mechanisms of the reagents.

RESULTS

Serum amylase assay and histology revealed that the severity of acute pancreatitis was reduced by the combinatory treatment with resveratrol and guggulsterone, but the ratio of the band intensity implied that reduced nuclear factor-κB activation is primarily responsible for the effect. The reduced amounts of keratinocyte chemoattractant (chemokine [C-X-C motif] ligand 1), interferon gamma-induced protein 10 (C-X-C motif chemokine 10) and interleukin 6 expression in the sera could be involved in attenuated immune cell migration and reduced inflammation by these reagents.

CONCLUSIONS

Combinatory treatment with resveratrol and guggulsterone marginally reduced cerulein-induced mild acute pancreatitis in mice.

摘要

目的

本研究旨在评估白藜芦醇和古古甾酮联合给药对小鼠雨蛙肽诱导的急性胰腺炎的预防/治疗作用。

方法

通过腹腔注射雨蛙肽诱导小鼠急性胰腺炎。进行血清淀粉酶测定和组织学检查以测量胰腺炎的严重程度。进行蛋白质免疫印迹和多重细胞因子/趋化因子分析以了解试剂的作用机制。

结果

血清淀粉酶测定和组织学检查显示，白藜芦醇和古古甾酮联合治疗可减轻急性胰腺炎的严重程度，但条带强度比值表明，核因子-κB活化的降低是该效应的主要原因。血清中角质形成细胞趋化因子（趋化因子[C-X-C基序]配体1）、γ干扰素诱导蛋白10（C-X-C基序趋化因子10）和白细胞介素6表达量的降低可能与这些试剂减弱免疫细胞迁移和减轻炎症有关。