Lin Ze-Si, Ku Chuen Fai, Guan Yi-Fu, Xiao Hai-Tao, Shi Xiao-Ke, Wang Hong-Qi, Bian Zhao-Xiang, Tsang Siu Wai, Zhang Hong-Jie
School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, China.
Phytother Res. 2016 Apr;30(4):663-70. doi: 10.1002/ptr.5576. Epub 2016 Feb 1.
Acute pancreatitis is an inflammatory process originated in the pancreas; however, it often leads to systemic complications that affect distant organs. Acute respiratory distress syndrome is indeed the predominant cause of death in patients with severe acute pancreatitis. In this study, we aimed to delineate the ameliorative effect of dihydro-resveratrol, a prominent analog of trans-resveratrol, against acute pancreatitis-associated lung injury and the underlying molecular actions. Acute pancreatitis was induced in rats with repetitive injections of cerulein (50 µg/kg/h) and a shot of lipopolysaccharide (7.5 mg/kg). By means of histological examination and biochemical assays, the severity of lung injury was assessed in the aspects of tissue damages, myeloperoxidase activity, and levels of pro-inflammatory cytokines. When treated with dihydro-resveratrol, pulmonary architectural distortion, hemorrhage, interstitial edema, and alveolar thickening were significantly reduced in rats with acute pancreatitis. In addition, the production of pro-inflammatory cytokines and the activity of myeloperoxidase in pulmonary tissues were notably repressed. Importantly, nuclear factor-kappaB (NF-κB) activation was attenuated. This study is the first to report the oral administration of dihydro-resveratrol ameliorated acute pancreatitis-associated lung injury via an inhibitory modulation of pro-inflammatory response, which was associated with a suppression of the NF-κB signaling pathway.
急性胰腺炎是一种起源于胰腺的炎症过程；然而，它常常导致影响远处器官的全身并发症。急性呼吸窘迫综合征确实是重症急性胰腺炎患者死亡的主要原因。在本研究中，我们旨在阐明白藜芦醇的一种重要类似物二氢白藜芦醇对急性胰腺炎相关肺损伤的改善作用及其潜在的分子作用机制。通过重复注射雨蛙素（50 µg/kg/h）和一剂脂多糖（7.5 mg/kg）诱导大鼠发生急性胰腺炎。通过组织学检查和生化分析，从组织损伤、髓过氧化物酶活性和促炎细胞因子水平等方面评估肺损伤的严重程度。用二氢白藜芦醇治疗后，急性胰腺炎大鼠的肺结构扭曲、出血、间质水肿和肺泡增厚明显减轻。此外，肺组织中促炎细胞因子的产生和髓过氧化物酶的活性显著受到抑制。重要的是，核因子-κB（NF-κB）的激活减弱。本研究首次报道口服二氢白藜芦醇通过抑制促炎反应改善急性胰腺炎相关肺损伤，这与抑制NF-κB信号通路有关。
