Sakao Masayuki, Sakaguchi Yutaku, Suzuki Ryo, Takahashi Yu, Kishimoto Masanobu, Fukui Toshiro, Uchida Kazushige, Nishio Akiyoshi, Matsuzaki Koichi, Okazaki Kazuichi
From the Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Pancreas. 2017 May/Jun;46(5):605-613. doi: 10.1097/MPA.0000000000000759.
The aims of this study are to characterize cell proliferation and differentiation during regeneration after pancreatitis and pancreatic buds during development to evaluate the role of Smad2/3, phosphorylated at the specific linker threonine residues (pSmad2/3L-Thr) in positive cells.
Male C57BL/6 mice received hourly intraperitoneal injections of cerulein and were analyzed after induced pancreatitis. Pancreatitis-affected tissue sections and pancreatic buds were immunostained for pSmad2/3L-Thr, with other markers thought to be stem/progenitor markers of the pancreas.
pSmad2/3L-Thr immunostaining-positive cells increased as the pancreatitis progressed. The expression of pSmad2/3L-Thr was seen in acinar cells and ductlike tubular complexes. These results suggest that pSmad2/3L-Thr is expressed during acinar-ductal metaplasia. Immunohistochemical colocalization of pSmad2/3L-Thr with Ki67 was never observed. pSmad2/3L-Thr-positive cells may remain in an undifferentiated state. During the pancreatic development process, pSmad2/3L-Thr was expressed as other markers. pSmad2/3L-Thr develops in duct structure of the undifferentiated cell population in the last part of viviparity that acinar structure is formed clearly.
pSmad2/3L-Thr expression occurs during acinar-ductal metaplasia after pancreatitis and may represent the contribution of stem cells and/or progenitor cells to the differentiation of the pancreas.
本研究旨在描述胰腺炎后再生过程中的细胞增殖和分化以及发育过程中的胰腺芽，以评估在特定连接苏氨酸残基处磷酸化的Smad2/3（pSmad2/3L-Thr）在阳性细胞中的作用。
雄性C57BL/6小鼠每小时接受腹腔注射蛙皮素，诱导胰腺炎后进行分析。对胰腺炎受累组织切片和胰腺芽进行pSmad2/3L-Thr免疫染色，并使用其他被认为是胰腺干细胞/祖细胞标志物的标记物。
随着胰腺炎的进展，pSmad2/3L-Thr免疫染色阳性细胞增多。在腺泡细胞和导管样管状复合体中可见pSmad2/3L-Thr的表达。这些结果表明pSmad2/3L-Thr在腺泡-导管化生过程中表达。从未观察到pSmad2/3L-Thr与Ki67的免疫组化共定位。pSmad2/3L-Thr阳性细胞可能保持未分化状态。在胰腺发育过程中，pSmad2/3L-Thr与其他标志物一起表达。pSmad2/3L-Thr在胎生后期未分化细胞群体的导管结构中发育，此时腺泡结构清晰形成。
pSmad2/3L-Thr表达发生在胰腺炎后的腺泡-导管化生过程中，可能代表干细胞和/或祖细胞对胰腺分化的贡献。
