Fialová Stanislava, Augustin Marco, Fischak Corinna, Schmetterer Leopold, Handschuh Stephan, Glösmann Martin, Pircher Michael, Hitzenberger Christoph K, Baumann Bernhard
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria; Department of Clinical Pharmacology, General Hospital and Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
Biomed Opt Express. 2016 Dec 16;8(1):298-314. doi: 10.1364/BOE.8.000298. eCollection 2017 Jan 1.
Polarization sensitive optical coherence tomography (PS-OCT) operating at 840 nm with axial resolution of 3.8 µm in tissue was used for investigating the posterior rat eye during an acute intraocular pressure (IOP) increase experiment. IOP was elevated in the eyes of anesthetized Sprague Dawley rats by cannulation of the anterior chamber. Three dimensional PS-OCT data sets were acquired at IOP levels between 14 mmHg and 105 mmHg. Maps of scleral birefringence, retinal nerve fiber layer (RNFL) retardation and relative RNFL/retina reflectivity were generated in the peripapillary area and quantitatively analyzed. All investigated parameters showed a substantial correlation with IOP. In the low IOP range of 14-45 mmHg only scleral birefringence showed statistically significant correlation. The polarization changes observed in the PS-OCT imaging study presented in this work suggest that birefringence of the sclera may be a promising IOP-related parameter to investigate.
采用在组织中轴向分辨率为3.8 µm、工作波长为840 nm的偏振敏感光学相干断层扫描（PS-OCT），在急性眼压（IOP）升高实验期间对大鼠的后眼进行研究。通过前房插管使麻醉的Sprague Dawley大鼠眼压升高。在14 mmHg至105 mmHg的眼压水平下采集三维PS-OCT数据集。在视乳头周围区域生成巩膜双折射、视网膜神经纤维层（RNFL）延迟和相对RNFL/视网膜反射率图，并进行定量分析。所有研究参数均与眼压显著相关。在14-45 mmHg的低眼压范围内，仅巩膜双折射显示出统计学上的显著相关性。本研究中PS-OCT成像研究观察到的偏振变化表明，巩膜双折射可能是一个有前景的与眼压相关的研究参数。
