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CAP-Gly蛋白通过与α-微管蛋白的C末端芳香族残基相互作用，参与微管依赖性运输。

CAP-Gly proteins contribute to microtubule-dependent trafficking via interactions with the C-terminal aromatic residue of α-tubulin.

作者信息

Andrieux Annie, Aubry Laurence, Boscheron Cécile

机构信息

a Université Grenoble Alpes , Grenoble , France.

b Inserm , U1216 , Grenoble , France.

出版信息

Small GTPases. 2019 Mar;10(2):138-145. doi: 10.1080/21541248.2016.1277002. Epub 2017 Jan 27.

DOI:10.1080/21541248.2016.1277002
PMID:28103137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6380331/
Abstract

In mammals, the C-terminal tyrosine residue of α-tubulin is subjected to removal/re-addition cycles resulting in tyrosinated microtubules and detyrosinated Glu-microtubules. CLIP170 and its yeast ortholog (Bik1) interact weakly with Glu-microtubules. Recently, we described a Microtubule- Rho1- and Bik1-dependent mechanism involved in Snc1 routing. Here, we further show a contribution of the yeast p150Glued ortholog (Nip100) in Snc1 trafficking. Both CLIP170 and p150Glued are CAP-Gly-containing proteins that belong to the microtubule +end-tracking protein family (known as +Tips). We discuss the +Tips-dependent role of microtubules in trafficking, the role of CAP-Gly proteins as possible molecular links between microtubules and vesicles, as well as the contribution of the Rho1-GTPase to the regulation of the +Tips repertoire and the partners associated with microtubules.

摘要

在哺乳动物中，α-微管蛋白的C末端酪氨酸残基会经历去除/重新添加循环，从而产生酪氨酸化微管和去酪氨酸化的谷氨酸化微管。CLIP170及其酵母同源物（Bik1）与谷氨酸化微管的相互作用较弱。最近，我们描述了一种参与Snc1分选的微管-Rho1-和Bik1依赖性机制。在此，我们进一步表明酵母p150Glued同源物（Nip100）在Snc1运输中发挥作用。CLIP170和p150Glued都是含CAP-Gly的蛋白质，属于微管正端追踪蛋白家族（称为+末端跟踪蛋白）。我们讨论了微管在运输中依赖于+末端跟踪蛋白的作用、CAP-Gly蛋白作为微管与囊泡之间可能的分子连接的作用，以及Rho1-GTP酶对+末端跟踪蛋白库和与微管相关的伙伴的调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69b7/6380331/21d98f36f442/ksgt-10-02-1277002-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69b7/6380331/66ac7b7b9744/ksgt-10-02-1277002-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69b7/6380331/21d98f36f442/ksgt-10-02-1277002-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69b7/6380331/66ac7b7b9744/ksgt-10-02-1277002-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69b7/6380331/21d98f36f442/ksgt-10-02-1277002-g002.jpg

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2
Invited review: Microtubule severing enzymes couple atpase activity with tubulin GTPase spring loading.特邀综述：微管切断酶将ATP酶活性与微管蛋白GTP酶弹簧加载耦合。
Biopolymers. 2016 Aug;105(8):547-56. doi: 10.1002/bip.22842.
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α-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons.
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Biol Open. 2019 Jan 29;8(1):bio038620. doi: 10.1242/bio.038620.
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Traffic. 2019 Mar;20(3):187-201. doi: 10.1111/tra.12629. Epub 2019 Jan 15.
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