a Faculty of Agriculture, Tokyo University of Agriculture, Funako , Atsugi , Kanagawa , Japan.
b Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Nishi-shirakawa , Fukushima , Japan.
Anim Biotechnol. 2017 Oct 2;28(4):242-247. doi: 10.1080/10495398.2016.1267643. Epub 2017 Jan 19.
Weak calf syndrome (WCS) is a major cause of calf death in Japanese Black cattle. Among IARS disorders, the isoleucyl-tRNA synthetase c.235G>C mutation has been identified as one of the causes of WCS. However, calf deaths differing from those attributed to IARS disorder has been occurring. To identify other genes potentially responsible for these calf deaths, we constructed three populations of three bulls (Bull-1, -2 and -3) that did not carry the IARS mutation, and dead calves (18, 28, and 31 calves) and healthy cattle (18, 15, and 10 cattle) sired by these bulls. The populations were genotyped using the BovineSNP50 BeadChip, but homozygosity mapping did not detect any associated genomic regions with calf death. Linkage analysis performed using each population as a paternal half-sib family of Bull-1, Bull-2, and Bull-3 revealed that, in the Bull-1 population, calf death was mapped to the 8.94 Mb-14.53 Mb and 29.82 Mb-33.77 Mb regions of BTA29. The findings suggested that the incidence of calf death in calves sired by Bull-1 was a hereditary disease exhibiting a dominant, not recessive, inheritance pattern.
弱牛综合征（WCS）是日本黑牛 calf 死亡的主要原因。在 IARS 障碍中，异亮氨酰-tRNA 合成酶 c.235G>C 突变已被确定为 WCS 的原因之一。然而，与归因于 IARS 障碍的 calf 死亡不同的 calf 死亡一直在发生。为了确定其他可能导致这些 calf 死亡的基因，我们构建了三个不携带 IARS 突变的公牛（公牛-1、-2 和-3）及其后代的三个群体：死亡 calf（18、28 和 31 头）和健康 calf（18、15 和 10 头）。使用 BovineSNP50 BeadChip 对这些群体进行了基因分型，但纯合性映射并未检测到与 calf 死亡相关的任何基因组区域。使用每个群体作为公牛-1、公牛-2 和公牛-3 的父系半同胞家系进行连锁分析表明，在公牛-1 群体中，calf 死亡被映射到 BTA29 的 8.94 Mb-14.53 Mb 和 29.82 Mb-33.77 Mb 区域。研究结果表明，公牛-1 所生 calf 的 calf 死亡发病率是一种显性遗传疾病，而非隐性遗传模式。
