近视性脉络膜新生血管患者眼内细胞因子及其与临床参数的相关性
Intraocular Cytokines and Their Correlations with Clinical Parameters in Patients with Myopic Choroidal Neovascularization.
作者信息
Shchuko Andrey G, Zaitseva Natalia V, Yurieva Tatyana N, Chernykh Elena R, Mikhalevich Isay M, Shevela Ekaterina Y, Grigorieva Alina V
机构信息
Irkutsk Branch of the Academician S.N. Fyodorov Eye Microsurgery Federal State Institution, Irkutsk, Russia.
出版信息
Ophthalmologica. 2017;237(2):96-104. doi: 10.1159/000455271. Epub 2017 Jan 20.
PURPOSE
To study cytokine levels in aqueous humor of patients with myopic choroidal neovascularization (mCNV), their correlations with each other and ocular parameters.
METHODS
Ophthalmological examination and immunological study of aqueous humor with cytokine levels measurement (Bio-Plex™ Human Cytokine 27-Plex panel; Bio-Rad Laboratories, USA) were performed in 19 patients (19 eyes) with ranibizumab-treated mCNV and compared to 15 patients (15 eyes) with myopia without CNV.
RESULTS
The levels of 10 cytokines were significantly different in patients with mCNV compared to the controls: the vascular endothelial growth factor (VEGF) level was 2 times lower (191.15 ± 142.30 and 320.06 ± 170.05 pg/mL, respectively), and the levels of PDGF, IL-2, IL-5, IL-13, IL-15, IL-17А, TNF-α, IL-8, and RANTES were elevated. Strong correlations between morphological and functional parameters and cytokines, including VEGF, were found. The VEGF level inversely correlated with the myopia degree and the cytokines IL-13, INF-γ, and RANTES.
CONCLUSION
The decrease in VEGF levels accompanied by imbalance of other cytokines may suggest additional mCNV development pathways.
目的
研究近视性脉络膜新生血管（mCNV）患者房水中的细胞因子水平、它们之间的相互关系以及眼部参数。
方法
对19例（19只眼）接受雷珠单抗治疗的mCNV患者进行眼科检查及房水免疫学研究并测量细胞因子水平（Bio-Plex™人类细胞因子27种检测试剂盒；美国伯乐公司），并与15例无CNV的近视患者（15只眼）进行比较。
结果
与对照组相比，mCNV患者中有10种细胞因子水平存在显著差异：血管内皮生长因子（VEGF）水平降低了2倍（分别为191.15±142.30和320.06±170.05 pg/mL），而血小板衍生生长因子（PDGF）、白细胞介素-2（IL-2）、白细胞介素-5（IL-5）、白细胞介素-13（IL-13）、白细胞介素-15（IL-15）、白细胞介素-17A（IL-17А）、肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）和调节激活正常T细胞表达和分泌因子（RANTES）水平升高。发现形态学和功能参数与包括VEGF在内的细胞因子之间存在强相关性。VEGF水平与近视度数以及细胞因子IL-13、干扰素-γ（INF-γ）和RANTES呈负相关。
结论
VEGF水平降低并伴有其他细胞因子失衡可能提示mCNV存在其他发病途径。
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