哇巴因诱导的大鼠和小鼠肠系膜淋巴结及胸腺细胞中ABCB1的改变。
Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice.
作者信息
Lima Daniel Boff, Valente Raphael Carmo, Capella Marcia Alves Marques
机构信息
Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21949-900, Brazil.
Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21949-900, Brazil.
出版信息
Oncol Lett. 2016 Dec;12(6):5275-5280. doi: 10.3892/ol.2016.5366. Epub 2016 Nov 8.
Ouabain is a glycoside with immunomodulating properties, and recent studies have suggested its use in adjuvant therapy for cancer treatment. Ouabain is known to modulate the immune system , and previous studies have revealed that ouabain can modulate the expression and activity of ABCB1, a protein associated with multidrug resistance present in immune system. Therefore, the present study investigated alterations in the expression and activity of ABCB1 in the thymi, peripheral blood monocytes and lymph nodes of Wistar rats and Swiss mice treated acutely or chronically with ouabain. A decrease of almost 45% in the monocyte count and an increase of 55% in the basophil count were observed. A significant decrease (75% reduction) in the amount of cells with ABCB1 activity was found in the thymocytes of ouabain-treated rats and mice. The possible implications of these results for cancer treatment are discussed.
哇巴因是一种具有免疫调节特性的糖苷，最近的研究表明其可用于癌症治疗的辅助疗法。已知哇巴因可调节免疫系统，先前的研究已揭示哇巴因可调节ABCB1的表达和活性，ABCB1是一种与免疫系统中存在的多药耐药性相关的蛋白质。因此，本研究调查了急性或慢性给予哇巴因的Wistar大鼠和瑞士小鼠的胸腺、外周血单核细胞和淋巴结中ABCB1的表达和活性变化。观察到单核细胞计数下降近45%，嗜碱性粒细胞计数增加55%。在给予哇巴因的大鼠和小鼠的胸腺细胞中发现具有ABCB1活性的细胞数量显著减少（减少75%）。讨论了这些结果对癌症治疗的可能影响。
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