Stephen Alexa A, Leone Angelique M, Toplon David E, Archer Linda L, Wellehan James F X
J Avian Med Surg. 2016 Dec;30(4):378-385. doi: 10.1647/2015-120.
A juvenile female bald eagle ( Haliaeetus leucocephalus ) was presented with emaciation and proliferative periocular lesions. The eagle did not respond to supportive therapy and was euthanatized. Histopathologic examination of the skin lesions revealed plaques of marked epidermal hyperplasia parakeratosis, marked acanthosis and spongiosis, and eosinophilic intracytoplasmic inclusion bodies. Novel polymerase chain reaction (PCR) assays were done to amplify and sequence DNA polymerase and rpo147 genes. The 4b gene was also analyzed by a previously developed assay. Bayesian and maximum likelihood phylogenetic analyses of the obtained sequences found it to be poxvirus of the genus Avipoxvirus and clustered with other raptor isolates. Better phylogenetic resolution was found in rpo147 rather than the commonly used DNA polymerase. The novel consensus rpo147 PCR assay will create more accurate phylogenic trees and allow better insight into poxvirus history.
一只幼年雌性白头海雕（Haliaeetus leucocephalus）出现消瘦和眼周增殖性病变。这只海雕对支持性治疗无反应，最终实施了安乐死。对皮肤病变进行组织病理学检查，发现有明显的表皮增生、角化不全、棘皮症和海绵形成斑块，以及嗜酸性胞质内包涵体。进行了新型聚合酶链反应（PCR）检测，以扩增和测序DNA聚合酶和rpo147基因。还通过先前开发的检测方法对4b基因进行了分析。对获得的序列进行贝叶斯和最大似然系统发育分析，发现它是禽痘病毒属的痘病毒，并与其他猛禽分离株聚集在一起。在rpo147基因中发现了比常用的DNA聚合酶更好的系统发育分辨率。新型共识rpo147 PCR检测将创建更准确的系统发育树，并有助于更好地了解痘病毒的历史。
