Guillaume Yves Claude, André Claire
University of Bourgogne Franche Comté, F-25000 Besançon, France; EA481 Neurosciences Intégratives et Cliniques/Pôle Chimie Analytique Bioanalytique et Physique (PCABP), F-25000 Besançon, France; CHRU Besançon, Pôle Pharmaceutique, F- 25000 Besançon, France.
University of Bourgogne Franche Comté, F-25000 Besançon, France; EA481 Neurosciences Intégratives et Cliniques/Pôle Chimie Analytique Bioanalytique et Physique (PCABP), F-25000 Besançon, France.
Talanta. 2017 Mar 1;164:39-44. doi: 10.1016/j.talanta.2016.10.105. Epub 2016 Nov 5.
In this paper, a novel and very simple homogeneous coating of a monolithic silica HPLC support using pristine boron nitride nanotubes (BNNTs) was d0.escribed. The chromatographic support was coated with BNNTs in a non covalent way to preserve the nanotube structure. A solution of BNNTs dispersed in dimethylacetamide (DMAc) was pumped through the column at a flow-rate of 0.3mL/min for 24h at room temperature. Strong interaction between amino groups and the BNNT surfaces induces the adsorption of the BNNTs on the silica, while the stable solvation in DMAc hampers further adsorption of the tubes. The excellent stability of the non covalent BNNT-coating on the monolithic silica in view of application for HPLC was also demonstrated. It was shown that this novel stationary phase was efficient for the HPLC isocratic or gradient mode separation of molecules of different structure such as phenol derivatives, alkylbenzene or doping agents (steroids). As well, this simple technique of BNNT immobilization offers new perspectives for the BNNT-coating on the surfaces of a wide range of solid substrates.
本文描述了一种使用原始氮化硼纳米管（BNNTs）对整体硅胶高效液相色谱（HPLC）载体进行新颖且非常简单的均相涂覆方法。色谱载体以非共价方式用BNNTs涂覆，以保留纳米管结构。将分散在二甲基乙酰胺（DMAc）中的BNNTs溶液在室温下以0.3mL/min的流速泵入柱中24小时。氨基与BNNT表面之间的强相互作用促使BNNTs吸附在硅胶上，而在DMAc中的稳定溶剂化作用则阻碍了纳米管的进一步吸附。还证明了这种非共价BNNT涂层在整体硅胶上用于HPLC时具有出色的稳定性。结果表明，这种新型固定相对不同结构的分子（如酚类衍生物、烷基苯或掺杂剂（类固醇））进行HPLC等度或梯度模式分离是有效的。同样，这种简单的BNNT固定技术为在各种固体基质表面进行BNNT涂覆提供了新的前景。
