Chen Shuisen, Ma Hui, Guo Zhifu, Feng Yaping, Lin Jingwei, Zhang Menghua, Zhong Ming
Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang, 110866, China.
Environ Sci Pollut Res Int. 2017 Mar;24(8):7375-7382. doi: 10.1007/s11356-017-8403-0. Epub 2017 Jan 20.
Petroleum is not only an important energy resource but is also a major soil pollutant. To gain better insight into the adaptability mechanism of Mirabilis jalapa to petroleum-contaminated soil, the protein profiles of M. jalapa root were investigated using label-free quantitative proteomics technique. After exposing to petroleum-contaminated soil for 24 h, 34 proteins significantly changed their protein abundance and most of the proteins increased in protein abundance (91.18%). Combined with gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses as well as data from previous studies, our results revealed that M. jalapa enhanced tolerance to petroleum by changing antioxidation and detoxification, cell wall organization, amino acid and carbohydrate metabolism, transportation and protein process, and so on. These metabolism alterations could result in the production and secretion of low molecular carbohydrate, amino acid, and functional protein, which enhanced the bioavailability of petroleum and reducing the toxicity of the petroleum. Taken together, these results provided novel information for better understanding of the tolerance of M. jalapa to petroleum stress.
石油不仅是一种重要的能源资源，也是一种主要的土壤污染物。为了更好地了解紫茉莉对石油污染土壤的适应机制，采用无标记定量蛋白质组学技术研究了紫茉莉根的蛋白质谱。在暴露于石油污染土壤24小时后，34种蛋白质的丰度发生了显著变化，且大多数蛋白质的丰度增加（91.18%）。结合基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析以及先前研究的数据，我们的结果表明，紫茉莉通过改变抗氧化和解毒、细胞壁组织、氨基酸和碳水化合物代谢、运输和蛋白质加工等过程来增强对石油的耐受性。这些代谢改变可能导致低分子碳水化合物、氨基酸和功能蛋白的产生和分泌，从而提高石油的生物可利用性并降低石油的毒性。综上所述，这些结果为更好地理解紫茉莉对石油胁迫的耐受性提供了新的信息。
