Pramanick Paulami, Chakraborty Anindita, Raychaudhuri Sarmistha Sen
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, APC Road, Kolkata, 700009, India.
Kolkata Centre, UGC- DAE Consortium for Scientific Research, LB- 8, Sector- III, Bidhan Nagar, Kolkata, 700098, India.
Biometals. 2017 Apr;30(2):171-184. doi: 10.1007/s10534-017-9990-4. Epub 2017 Jan 20.
Plantago ovata Forsk is an annual herb with immense medicinal importance, the seed and husk of which is used in the treatment of chronic constipation, irritable bowel syndrome, diarrhea since ancient times. Zinc, an essential metal, is required by plants as they form important components of zinc finger proteins and also aid in synthesis of photosynthetic pigments such as chlorophyll. However, in excess amount Zn causes chlorosis of leaf and shoot tissues and generate reactive oxygen species. The present study is aimed at investigating the changes in expression levels of MT2 gene in Plantago ovata under zinc stress. Data show up to 1.66 fold increase in expression of PoMT2 in 1000 µM ZnSO·7HO treated sample. Our study also describes alteration of MT2 gene expressions in Plantago ovata as observed through Real time PCR (qPCR) done by [Formula: see text] method. In this study we have observed an upregulation (or induction) in the PoMT2 gene expression level in 500 and 800 µM ZnSO·7HO treated samples but found saturation on further increasing the dose to 1000 µM of ZnSO·7HO. Determination of the phenotypic and biochemical changes in Plantago ovata due to exposure to zinc stress of concentrations 500, 800 and 1000 µM revealed oxidative stress. The enhanced expression of MT2 gene in Plantago ovata has a correlation with the increased total antioxidant activity and increased DPPH radical scavenging activity.
卵叶车前是一种具有重要药用价值的一年生草本植物，其种子和种皮自古以来就被用于治疗慢性便秘、肠易激综合征和腹泻。锌是一种必需金属，植物需要它来形成锌指蛋白的重要成分，还能帮助合成叶绿素等光合色素。然而，过量的锌会导致叶片和茎组织黄化，并产生活性氧。本研究旨在调查锌胁迫下卵叶车前MT2基因表达水平的变化。数据显示，在1000 μM ZnSO₄·7H₂O处理的样品中，PoMT2的表达增加了1.66倍。我们的研究还描述了通过[公式：见文本]方法进行的实时PCR（qPCR）观察到的卵叶车前MT2基因表达的变化。在本研究中，我们观察到在500和800 μM ZnSO₄·7H₂O处理的样品中，PoMT2基因表达水平上调（或诱导），但在将剂量进一步增加到1000 μM ZnSO₄·7H₂O时发现达到饱和。对卵叶车前暴露于500、800和1000 μM浓度锌胁迫下的表型和生化变化的测定显示出氧化应激。卵叶车前中MT2基因的增强表达与总抗氧化活性增加和DPPH自由基清除活性增加相关。
