Veillon L, Zhou S, Mechref Y
Texas Tech University, Lubbock, TX, United States.
Texas Tech University, Lubbock, TX, United States.
Methods Enzymol. 2017;585:431-477. doi: 10.1016/bs.mie.2016.11.006. Epub 2017 Jan 9.
Glycosylation is one of the most common and essential protein modifications. Glycans conjugated to biomolecules modulate the function of such molecules through both direct recognition of glycan structures and indirect mechanisms that involve the control of protein turnover rates, stability, and conformation. The biological attributes of glycans in numerous biological processes and implications in a number of diseases highlight the necessity for comprehensive characterization of protein glycosylation. This chapter reviews cutting-edge methods and tools developed to facilitate quantitative glycomics. This chapter highlights the different methods employed for the release and purification of glycans from biological samples. The most effective labeling methods developed for sensitive quantitative glycomics are also described and discussed. The chromatographic approaches that have been used effectively in glycomics are also highlighted.
糖基化是最常见且重要的蛋白质修饰之一。与生物分子共轭的聚糖通过对聚糖结构的直接识别以及涉及控制蛋白质周转率、稳定性和构象的间接机制来调节此类分子的功能。聚糖在众多生物过程中的生物学特性以及在多种疾病中的影响凸显了全面表征蛋白质糖基化的必要性。本章综述了为促进定量糖组学而开发的前沿方法和工具。本章重点介绍了从生物样品中释放和纯化聚糖所采用的不同方法。还描述并讨论了为灵敏的定量糖组学而开发的最有效的标记方法。同时也强调了在糖组学中有效使用的色谱方法。
