Wang Yun-Qi, Yu Shi-Jin, Zhang Fang, Xia Xiu-Yang, Zeng Raymond J
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, People's Republic of China.
School of Resources and Environment, Anqing Normal University, Anqing, Anhui, China.
Appl Microbiol Biotechnol. 2017 Mar;101(6):2619-2627. doi: 10.1007/s00253-017-8124-9. Epub 2017 Jan 21.
Conversion of organic wastes to syngas is an attractive way to utilize wastes. The produced syngas can be further used to produce a variety of chemicals. In this study, a hollow-fiber membrane biofilm reactor with mix cultures was operated at 55 °C to convert syngas (H/CO) into acetate. A high concentration of acetate (42.4 g/L) was reached in batch experiment while a maximum acetate production rate of 10.5 g/L/day was achieved in the continuous-flow mode at hydraulic retention time (HRT) of 1 day. Acetate was the main product in both batch and continuous-flow experiments. n-Butyrate was the other byproduct in the reactor. Acetate accounted for more than 98.5 and 99.1% of total volatile fatty acids in batch and continuous modes, respectively. Illumina Miseq high-throughput sequencing results showed that microorganisms were highly purified and enriched in the reactor. The main genus was Thermoanaerobacterium (66% of relative abundance), which was usually considered as H producer in the literature, however, likely played a role as a H consumer in this study. This study provides a new method to generate the high producing rate and purity of acetate from syngas.
将有机废物转化为合成气是一种颇具吸引力的废物利用方式。所产生的合成气可进一步用于生产各种化学品。在本研究中，一个装有混合培养物的中空纤维膜生物膜反应器在55°C下运行，将合成气（H/CO）转化为乙酸盐。在分批实验中达到了高浓度的乙酸盐（42.4 g/L），而在连续流模式下，当水力停留时间（HRT）为1天时，乙酸盐的最大生产率达到了10.5 g/L/天。乙酸盐是分批和连续流实验中的主要产物。丁酸盐是反应器中的另一种副产物。在分批和连续模式下，乙酸盐分别占总挥发性脂肪酸的98.5%和99.1%以上。Illumina Miseq高通量测序结果表明，反应器中的微生物得到了高度纯化和富集。主要菌属是嗜热厌氧菌（相对丰度为66%），在文献中通常被认为是氢气产生菌，然而，在本研究中它可能起到了氢气消耗菌的作用。本研究提供了一种从合成气中高产率、高纯度地生成乙酸盐的新方法。
